Anti-CYB5A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, and ELISA - P00167

Core Product Specifications and Parameters

Biological Background: CYB5A Function and Localization

• Cytochrome b5 (CYB5A), also known as microsomal cytochrome b5 type A (MCB5), is a membrane-bound hemoprotein that functions as an electron carrier for several membrane-bound oxygenases.
• The protein contains a heme-binding domain that facilitates electron transfer, essential for processes such as fatty acid desaturation and drug metabolism.
• CYB5A is encoded by the CYB5A gene (Gene ID: 1528) and is subject to alternative splicing, as indicated by proteomics studies.
• Subcellular localization includes the endoplasmic reticulum membrane, microsome membrane, and cytoplasm, consistent with its role in electron transport chains.
• Post-translational modifications of CYB5A include acetylation, identified through direct protein sequencing and proteomics.
• Key structural features include a transmembrane domain and metal-binding sites for heme and iron, critical for its redox function.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against the N-terminal region (amino acids 1-100) of human CYB5A; consider its suitability for detecting full-length and truncated forms in Western blotting.
• For WB, validate the antibody in relevant cell lines or tissues expressing CYB5A, and optimize loading controls based on the predicted molecular weight of ~15 kDa.
• In IHC-P and IF/ICC, note the membrane and cytoplasmic localization; adjust fixation and permeabilization conditions accordingly. Antigen retrieval may be required for paraffin-embedded sections.
• Cross-reactivity with mouse and rat is reported; verify in your specific sample system using appropriate positive and negative controls.
• As a polyclonal antibody, expect some batch-to-batch variability; we recommend titrating the antibody for each application and including a standard curve in ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with streamlined access to IVD raw materials, including validated antibodies like this CYB5A polyclonal antibody for studying electron transport and membrane biology. Our platform offers a comprehensive catalog of monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents, along with customized sourcing for rare targets, supporting your projects from concept to clinical application.