Anti-CXXC1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9P0U4

Core Product Specifications and Parameters

Biological Background: CXXC1 Function and Localization

• CXXC-type zinc finger protein 1 (CXXC1), also designated CpG-binding protein (CGBP) and PHD finger and CXXC domain-containing protein 1, is encoded by the CXXC1 gene in humans.
• It acts as a transcriptional activator that displays a unique binding preference for unmethylated CpG DNA motifs, particularly CpGG sequences.
• The protein contains characteristic CXXC-type zinc finger and PHD finger domains, which facilitate DNA binding and mediate protein-protein interactions.
• CXXC1 localizes predominantly to nuclear speckles and the nucleoplasm, consistent with its role in transcription regulation.
• Expression of CXXC1 is ubiquitous across human tissues, reflecting its fundamental involvement in chromatin and gene expression.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, suggesting multiple layers of functional regulation.
• The UniProt record also highlights alternative splicing, coiled-coil regions, and metal-binding properties, further underscoring its structural and functional versatility.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 401–660 of human CXXC1, a region that encompasses important functional domains; the resulting polyclonal antibody is likely to recognize both native and denatured protein.
• For Western blot, the predicted molecular weight is approximately 76 kDa (75712 Da per UniProt), but minor variations may occur due to alternative splicing or post-translational modifications.
• In immunofluorescence or immunocytochemistry, anticipate punctate nuclear staining, consistent with the protein’s localization to nuclear speckles; always include appropriate negative controls.
• ELISA performance is supported by the recombinant immunogen design; sandwich ELISA development may require pairing with a second validated antibody against a non-overlapping epitope.
• The antibody is stated to cross-react with mouse and rat; verify sequence homology in the immunogen region to assess potential reactivity with other species.
• For all applications, titration and optimization in the relevant sample matrix are recommended to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as an integrated procurement partner, streamlining access to a broad portfolio of IVD raw materials for diagnostic developers and life science researchers. This CXXC1 polyclonal antibody complements our offerings in epigenetics and transcriptional regulation, and we can additionally supply validated antibody pairs, optimized monoclonal reagents, and bulk ancillary materials. For targets with limited commercial availability, our team assists with customized sourcing to help bridge the gap from concept to clinical application.