Anti-CXXC1 Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q9P0U4

Core Product Specifications and Parameters

Biological Background: CXXC-type zinc finger protein 1 (CXXC1) Function and Localization

• CXXC1 is a transcriptional activator that specifically binds unmethylated CpG dinucleotides, with a preference for CpGG motifs, thereby regulating gene expression.
• It belongs to the CXXC-type zinc finger protein family, containing a CXXC domain that recognizes non-methylated DNA and a PHD finger domain.
• The protein localizes to nuclear speckles and the nucleoplasm, consistent with its role in transcription regulation.
• CXXC1 undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination, which may modulate its activity.
• It is ubiquitously expressed, suggesting a fundamental housekeeping function in chromatin and transcription.
• Alternative splicing produces isoforms that may fine-tune its function.
• The protein contains a coiled-coil region and can form isopeptide bonds, indicating potential protein-protein interactions.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-210) containing the CXXC domain, ensuring specific recognition of full-length CXXC1.
• For WB, consider using whole cell lysates from human or mouse cell lines; the predicted molecular weight is ~76 kDa.
• For IHC-P, validate antibody specificity using appropriate controls such as peptide blocking or tissue with known expression patterns.
• For IP, optimize antibody concentration and cross-linking conditions to capture the protein complex.
• For ELISA, coat the recombinant protein or synthetic peptide to evaluate binding affinity.
• Since CXXC1 is ubiquitously expressed, use positive controls such as HeLa, HEK293, or NIH/3T3 cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutions from concept to clinic. This anti-CXXC1 polyclonal antibody is part of our extensive portfolio for transcription and chromatin research applications. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate your assay development and scale-up.