Anti-CXCR6 Rabbit Polyclonal Antibody for WB and ELISA - O00574

Core Product Specifications and Parameters

Biological Background: CXCR6 Function and Localization

• CXCR6 is a C-X-C chemokine receptor that specifically binds to the chemokine CXCL16.
• It functions as a coreceptor for simian immunodeficiency viruses (SIVs) and certain strains of HIV-2 and m-tropic HIV-1.
• The receptor is a G protein-coupled receptor (GPCR) that transduces signals through heterotrimeric G proteins upon ligand binding.
• It is localized primarily on the cell membrane, consistent with its role as a seven-transmembrane domain receptor.
• Key structural features include disulfide bonds and glycosylation sites essential for receptor stability and function.
• CXCR6 is predominantly expressed in lymphoid tissues and on activated T cells, implicating its role in immune cell trafficking and homeostasis.
• The human CXCR6 gene is located at chromosome 3, and the encoded protein has a molecular weight of approximately 39 kDa (calculated from sequence 39.2 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain of human CXCR6, which is likely accessible for antibody binding in native conformations. For WB, a band around 39 kDa is expected; however, consider that glycosylation may result in a higher molecular weight band.
• For ELISA, the antibody may recognize both native and denatured protein; optimization of coating conditions is recommended.
• Since CXCR6 is a membrane receptor, ensure proper protein extraction and avoid harsh detergents that might disrupt epitope recognition.
• Cross-reactivity is reported only for human; perform appropriate controls if used in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials, including this anti-CXCR6 antibody for chemokine receptor and HIV coreceptor studies. As a comprehensive sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for rare target raw-material procurement, empowering your workflow from concept to clinic.