Anti-CXCR1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P25024

Core Product Specifications and Parameters

Biological Background: CXCR1 Function and Localization

• CXCR1, also known as CD181, CDw128a, IL-8 receptor type 1 (IL8RA), and CMKAR1, is a C-X-C chemokine receptor belonging to the G protein-coupled receptor (GPCR) family.
• It functions as a high-affinity receptor for interleukin-8 (IL-8), a potent chemoattractant for neutrophils, triggering cell migration to sites of inflammation (PubMed:1840701). Related references: PMID:1840701
• Ligand binding activates a G protein-mediated signaling cascade, including the phosphatidylinositol-calcium second messenger system, leading to neutrophil activation (PubMed:8662698). Related references: PMID:8662698
• CXCR1 is a plasma membrane protein with seven transmembrane domains, typical of GPCRs, and contains disulfide bonds and glycosylation sites important for its structure and function.
• It plays a pivotal role in innate immunity and inflammatory responses by directing neutrophil trafficking and activation.
• The protein is encoded by the CXCR1 gene (GeneID: 3577) located on human chromosome 2.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a cytoplasmic region (aa 250-350), which may be accessible in denaturing Western blot conditions; consider using reducing SDS-PAGE for optimal detection.
• For immunohistochemistry on paraffin-embedded sections, heat-induced antigen retrieval in an appropriate buffer may enhance epitope exposure.
• Validate the antibody in your specific sample system; expression levels of CXCR1 can vary, so consider using positive controls such as neutrophil lysates.
• The antibody reacts with human, mouse, and rat samples, suggesting recognition of a conserved epitope; cross-reactivity in other species should be verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with seamless access to a broad portfolio of IVD raw materials, including high-quality antibodies like this CXCR1 rabbit polyclonal antibody. Whether you require validated antibody pairs, bulk ancillary reagents, or assistance sourcing rare targets, our integrated supply chain supports your assay development from concept to clinic.