Anti-CXCL9 Rabbit Polyclonal Antibody for WB and ELISA - Q07325

Core Product Specifications and Parameters

Biological Background: CXCL9 Function and Localization

• CXCL9, also known as monokine induced by interferon-gamma (MIG), is a member of the CXC chemokine family and contains conserved cysteine residues that form disulfide bonds essential for its structure.
• It functions as a cytokine that affects the growth, movement, and activation of immune cells, acting as a potent chemoattractant for activated T lymphocytes.
• CXCL9 mediates its effects by binding to the chemokine receptor CXCR3, a G protein-coupled receptor expressed on activated T cells and other immune cells.
• The protein is secreted and plays a key role in inflammatory responses, with expression strongly induced by interferon-gamma.
• The mature protein has a calculated molecular mass of approximately 14 kDa, consistent with its small cytokine nature.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 50 to the C-terminus of human CXCL9; epitope mapping may be considered if detailed binding characteristics are required.
• For Western blot, a band near 14 kDa is expected; validate antibody performance in appropriate positive control samples such as stimulated immune cells or tissues expressing CXCL9.
• In ELISA applications, this antibody may be used for capture or detection; however, optimal pairing with a suitable monoclonal antibody or recombinant antigen is recommended for sandwich formats.
• Cross-reactivity with human CXCL9 is confirmed; reactivity with other species or related chemokines should be independently tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material supplier catering to diagnostic manufacturers, laboratories, and research institutes. This anti-CXCL9 polyclonal antibody is part of our extensive portfolio supporting chemokine biomarker research and immunoassay development. Beyond antibodies, CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized raw-material sourcing to accelerate your project from concept to clinic.