Anti-CXCL6 Rabbit Polyclonal Antibody (WB, ELISA) - P80162

Core Product Specifications and Parameters

Biological Background: CXCL6 Function and Localization

• CXCL6 (C-X-C motif chemokine 6) is a secreted chemokine belonging to the intercrine alpha (chemokine CXC) family.
• Alternative names include granulocyte chemotactic protein 2 (GCP-2), chemokine alpha 3 (CKA-3), and small-inducible cytokine B6 (SCYB6).
• It acts as a chemoattractant for neutrophil granulocytes, playing a critical role in inflammatory responses.
• Signaling occurs through high-affinity binding to CXCR1 and CXCR2 receptors, which also mediate angiogenic effects.
• Notably, CXCL6 possesses direct antibacterial activity against both Gram-positive and Gram-negative bacteria, at levels 90-fold higher than CXCL5 and CXCL7.
• The protein is characterized by a signal peptide, disulfide bond architecture, and a heparin-binding domain, consistent with its secretory and extracellular matrix-interacting functions.
• UniProt keywords highlight its roles in antibiotic, antimicrobial, chemotaxis, and cytokine signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 38–114 of human CXCL6, covering the mature secreted protein. Therefore, the antibody is expected to detect the processed, biologically active form.
• For Western blot, a band around 12 kDa is anticipated. Due to the presence of disulfide bonds, reducing conditions may be necessary; consider adding reducing agents to the sample buffer.
• Given its polyclonal nature, the antibody may exhibit broad reactivity; optimization of dilution and blocking conditions is recommended for each assay.
• The antibody cross-reacts with mouse CXCL6, enabling its use in mouse tissue or cell line studies; however, validation in the specific experimental system is essential.
• For ELISA applications, the polyclonal antibody can be used as a capture or detection reagent; pairing with a monoclonal antibody may enhance specificity.
• Since CXCL6 is secreted, sample preparation for cell culture supernatants or biological fluids should concentrate the protein if expression levels are low.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibodies for chemokine targets like CXCL6. Our comprehensive portfolio supports inflammation and immunology assay development with bulk quantities, optimized antibody pairs, and customized sourcing for niche biomarkers.