Anti-CXCL4/PF4 Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P02776

Core Product Specifications and Parameters

Biological Background: CXCL4/PF4 Function and Localization

• PF4 (also known as CXCL4, Iroplact, or Oncostatin-A) is a secreted C-X-C motif chemokine released during platelet aggregation.
• It regulates diverse biological processes including hematopoiesis, cell proliferation, differentiation, and activation. Related references: PMID:29930254, PMID:9531587
• PF4 signals via CCR1, CXCR3A, and CXCR3B receptors, mediating T-lymphocyte migration through Ras/ERK signaling. Related references: PMID:18174362, PMID:24469069, PMID:29930254
• It potently neutralizes the anticoagulant effect of heparin through high-affinity binding.
• PF4 plays a role in inhibiting hematopoiesis and maintaining hematopoietic stem cell quiescence. Related references: PMID:9531587
• The chemokine acts as a chemoattractant for neutrophils and monocytes via CCR1. Related references: PMID:29930254
• PF4 inhibits endothelial cell proliferation and, in cooperation with TLR8, induces chromatin remodeling and inflammatory gene expression via the TBK1-IRF5 axis. Related references: PMID:35701499
• It stimulates myofibroblast differentiation and collagen synthesis by promoting endothelial-to-mesenchymal transition. Related references: PMID:34986347
• Interaction with thrombomodulin enhances protein C activation, thus potentiating its anticoagulant activity. Related references: PMID:9395524

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length mature PF4 protein, suggesting the antibody may recognize both monomeric and multimeric forms.
• For Western blot (WB), consider analyzing under reducing and non-reducing conditions, as PF4 can form tetramers and other oligomers.
• This antibody is validated for IHC-P and IF-P; ensure appropriate antigen retrieval for paraffin sections and fixatives for IF.
• When using for ELISA, validate the antibody pair compatibility with the target epitope.
• Cross-reactivity with mouse and rat indicates potential for translational studies; verify in appropriate sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, covering every stage from concept to clinic. This anti-CXCL4/PF4 monoclonal antibody supports research into chemokine signaling, platelet biology, and hemostasis. Beyond this product, we offer validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and rare target sourcing to accelerate your assay development.