Anti-CXCL12 Polyclonal Antibody for WB - P48061

Core Product Specifications and Parameters

Biological Background: CXCL12 Function and Localization

• CXCL12 (Stromal cell-derived factor 1) is a chemokine of the C-X-C family, with multiple isoforms (Alpha, Beta, Gamma, Delta, Epsilon, Theta) generated by alternative splicing.
• It functions as a chemoattractant for T-lymphocytes and monocytes, but not neutrophils, signaling through CXCR4 to induce calcium flux and chemotaxis. Related references: PMID:8752281, PMID:18802065, PMID:39093700
• CXCL12 also binds the atypical receptor ACKR3, activating beta-arrestin pathways, acting as a scavenger receptor, and inducing down-regulation and internalization of ACKR3. Related references: PMID:16107333, PMID:19255243
• Through an allosteric site on integrins, CXCL12 can activate ITGAV:ITGB3, ITGA4:ITGB1, and ITGA5:ITGB1 independently of CXCR4. Related references: PMID:29301984
• It positively regulates monocyte migration while negatively regulating monocyte adhesion via LYN kinase, inhibiting LFA-1-mediated adhesion to ICAM-1. Related references: PMID:18802065
• CXCL12 inhibits HIV-1 infection of T-cells by competing with the virus for CXCR4 binding. Related references: PMID:8752281
• During embryonic development, it is required for B-cell lymphopoiesis, myelopoiesis, and heart ventricular septum formation (By similarity).
• The protein is secreted and ubiquitously expressed, with highest levels in liver, pancreas, and spleen; isoform Gamma is predominant in heart.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide (aa 40-93), suggesting that the antibody may recognize linear epitopes; consider denaturing conditions for Western blotting.
• For immunofluorescence and immunohistochemistry, validation on relevant tissue or cell samples is recommended to confirm specificity.
• Cross-reactivity with mouse and rat CXCL12 has been reported; verify performance in the desired species prior to assay setup.
• For ELISA applications, optimize coating antigen and antibody concentrations for sandwich or direct formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a one-stop solution for IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This anti-CXCL12 antibody is part of our broad chemokine and immunology portfolio, and we can also source rare targets to accelerate your assay development from concept to clinic.