Anti-CXCL10/IP-10 Rabbit Monoclonal Antibody for WB and ELISA - P02778

Core Product Specifications and Parameters

Biological Background: CXCL10 Function and Localization

• CXCL10, also known as IP-10, is a pro-inflammatory CXC chemokine crucial for immune cell chemotaxis, differentiation, and activation during inflammatory responses. Related references: PMID:11157474 PMID:22652417 PMID:7540647
• It facilitates the recruitment of activated Th1 lymphocytes to inflammatory sites via CXCR3 binding, which is critical for antiviral immunity. Related references: PMID:12663757 PMID:12750173
• Binding to CXCR3 activates G protein-mediated signaling, leading to increased intracellular calcium flux, actin polymerization, and phospholipase C pathway activation. Related references: PMID:12750173 PMID:19151743
• Primarily secreted by monocytes, endothelial cells, and fibroblasts; expression in thymic epithelium and microglia is strongly induced upon viral stimulation. Related references: PMID:11157474 PMID:12663757
• In the CNS, the CXCL10/CXCR3 axis orchestrates microglial migration to injury sites, a key step in neuronal repair and reorganization.
• Structural features include an N-terminal signal peptide for secretion, two conserved disulfide bonds characteristic of CXC chemokines, and post-translational citrullination.
• The predicted molecular weight of mature CXCL10 is approximately 10.9 kDa; this antibody detects an 11 kDa band consistent with the glycosylated or unmodified form in SDS-PAGE under reducing conditions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CXCL10 protein (aa 1-98, NP_001556.2), covering the secreted chemokine domain. This design supports recognition of both recombinant and endogenous CXCL10.
• For Western blotting, use reducing SDS-PAGE conditions and load appropriate positive control lysates (e.g., IFN-γ-stimulated human fibroblasts or monocytes). Expect a single band at ~11 kDa; consider using a low-molecular-weight marker for accurate sizing.
• For ELISA, this monoclonal antibody may be employed as a capture or detection reagent following standard sandwich ELISA optimization. Validate pairing with a compatible anti-CXCL10 antibody for your specific assay format.
• Due to the antibody’s human specificity, cross-reactivity with mouse or rat CXCL10 is not expected; verify in your experimental system if species cross-reactivity is required.
• Include appropriate blocking and washing steps to minimize background, especially when using chemiluminescent or colorimetric detection systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD manufacturers and research laboratories, offering a comprehensive portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents. This anti-CXCL10 monoclonal antibody exemplifies our commitment to providing high-quality raw materials for inflammation and immunology assay development. Through our global supply network, we can source rare-target antibodies, bulk reagents, and customized protein/antibody services to accelerate your diagnostic projects from concept to clinic.