Anti-CXCL10/IP-10 Polyclonal Antibody for WB, ELISA - P02778

Core Product Specifications and Parameters

Biological Background: C-X-C motif chemokine 10 (CXCL10) Function and Localization

• C-X-C motif chemokine 10 (CXCL10), also known as 10 kDa interferon gamma-induced protein (Gamma-IP10, IP-10) or Small-inducible cytokine B10, is a secreted cytokine encoded by the CXCL10 gene (Gene ID: 3627).
• Functions as a pro-inflammatory cytokine, regulating chemotaxis, differentiation, and activation of peripheral immune cells, as well as cell growth, apoptosis, and angiostatic effects [PMID:11157474, PMID:22652417, PMID:7540647]. Related references: PMID:11157474 PMID:22652417 PMID:7540647
• Binds to the CXCR3 receptor, activating G protein-mediated signaling that increases intracellular calcium and leads to actin reorganization, ultimately promoting chemotaxis of activated Th1 lymphocytes to inflammation sites [PMID:12750173, PMID:19151743, PMID:12663757]. Related references: PMID:12750173 PMID:19151743 PMID:12663757
• In the central nervous system, the CXCL10/CXCR3 axis mediates microglial activation and recruitment following brain injury, which is essential for neuronal reorganization (By similarity).
• Mainly secreted by monocytes, endothelial cells, and fibroblasts; also expressed in thymic epithelial cells and microglial cells upon viral stimulation [PMID:11157474, PMID:12663757]. Related references: PMID:11157474 PMID:12663757
• Contains a signal peptide and a disulfide bond, and is subject to citrullination; classified under inflammatory response, chemotaxis, and secreted proteins (UniProt keyword).

Experimental Guidance and Technical Tips

• For Western blotting, the expected molecular weight is approximately 11 kDa; the immunogen corresponds to the mature peptide (aa 20-98) without the signal peptide, so detection of secreted forms may require non-reducing conditions or careful sample preparation.
• As a secreted cytokine, CXCL10 is best analyzed in conditioned media, serum, or tissue extracts from activated immune cells; consider inducing expression with interferon-gamma or viral stimuli in appropriate cell lines.
• For ELISA applications, pairing this polyclonal antibody as either capture or detector with a well-characterized monoclonal can improve specificity; validate in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement channel for IVD raw materials, including validated polyclonal antibodies, monoclonal antibodies, and antibody pairs for chemokine targets like CXCL10. Whether you require bulk quantities, custom conjugations, or ancillary reagents, our supply chain expertise supports assay development from research to commercialization. Reach out to explore integrated sourcing solutions that accelerate your diagnostic projects.