Anti-CX3CR1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P49238

Core Product Specifications and Parameters

Biological Background: CX3CR1 Function and Localization

• CX3CR1, also known as fractalkine receptor, CMK-BRL1, or V28, is a G protein-coupled receptor that binds the chemokine fractalkine (CX3CL1). Related references: PMID:12055230, PMID:23125415, PMID:9390561
• The CX3CR1-CX3CL1 axis mediates diverse functions including immune cell migration, adhesion, and inflammatory responses across different tissue compartments.
• CX3CR1 is critical for leukocyte trafficking, such as NK cell recruitment to inflamed tissues and monocyte migration to non-inflamed tissues for differentiation into macrophages and dendritic cells.
• In the central nervous system, CX3CR1 regulates microglial inflammatory responses, synaptic pruning during brain development, and synapse maturation.
• CX3CR1 acts as a negative regulator of angiogenesis, likely through macrophage chemotaxis, and is involved in atherogenesis by recruiting monocytes to atherosclerotic plaques. Related references: PMID:14581400, PMID:18971423
• The receptor is expressed on lymphocyte subsets including NK cells, gamma-delta T-cells, and CD8+ T-cells, as well as in smooth muscle cells in atherosclerotic plaques. Related references: PMID:7590284, PMID:12055230, PMID:14581400
• CX3CR1 serves as a coreceptor with CD4 for HIV-1 virus entry, with isoform variants exhibiting differential activity. Related references: PMID:14607932
• Additionally, CX3CR1 plays a role in gut immunity by controlling microbiota defense and antifungal IgG responses, and it also functions as a receptor for CCL26 inducing chemotaxis. Related references: PMID:29326275, PMID:20974991

Experimental Guidance and Technical Tips

• For Western blotting, a band corresponding to the 40 kDa CX3CR1 protein may be observed; verify specificity using appropriate cell lines or tissues known to express CX3CR1.
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval conditions should be optimized for each sample type; consider testing citrate or EDTA-based retrieval buffers.
• As a polyclonal antibody, lot-to-lot consistency can vary; it is advisable to validate each new batch in the intended application with relevant positive and negative controls.
• The immunogen corresponds to an internal sequence (amino acids 100-200); thus, the antibody may recognize both full-length and some truncated or alternative splice variants that retain this region.
• For ELISA applications, pair this antibody with an appropriate capture or detection antibody; confirm reactivity with recombinant or native CX3CR1 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, empowering diagnostic manufacturers and researchers with validated antibodies, antigen pairs, and ancillary reagents. Our CX3CR1 polyclonal antibody supports studies in chemokine signaling, inflammation, and neuroimmunology, while we also offer monoclonal alternatives, bulk quantities, and custom development services to accelerate your project from concept to clinic. Let CamelBio bridge your supply chain with reliable, high-quality raw materials tailored to your diagnostic development needs.