Anti-CX3CL1 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - O35188

Core Product Specifications and Parameters

Biological Background: Fractalkine (CX3CL1) Function and Localization

• CX3CL1 (Fractalkine) is a C-X3-C motif chemokine that acts as a ligand for CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1, mediating immune response, inflammation, cell adhesion, and chemotaxis.
  Related references: PMID:10187784 PMID:18971423
• Through CX3CR1 signaling, CX3CL1 regulates leukocyte adhesion and migration at the endothelium, and can activate integrins in both a CX3CR1-dependent and -independent manner.
  Related references: PMID:10382755 PMID:9177350
• The membrane-bound form promotes adhesion of leukocytes to endothelial cells, while the proteolytically released soluble form is chemotactic for T-cells and monocytes, but not neutrophils.
• Fractalkine is predominantly expressed in the brain, with lower levels in kidney, heart, lung, skeletal muscle, and testis; it is also found in dendritic cells and stimulated B‑cells.
  Related references: PMID:10508268 PMID:9177350 PMID:9479488 PMID:9845323 PMID:12569158
• The protein is a type I transmembrane protein containing a signal peptide, a mucin‑like stalk, a transmembrane helix, and a short cytoplasmic tail; it can be cleaved to release the soluble chemokine domain.
• The chemokine domain is stabilized by conserved disulfide bonds, consistent with its classification in the C‑X3‑C subfamily.

Experimental Guidance and Technical Tips

• The immunogen (aa 25–337) spans the extracellular chemokine domain and part of the mucin stalk; the antibody may detect full‑length (~42 kDa) and cleaved/shed forms in WB.
• For IHC‑P and IF‑P, expect membrane‑localized staining; optimize fixation and permeabilization conditions for mouse tissues or cell lines.
• In ELISA, the antibody can be used to capture or detect soluble fractalkine in cell culture supernatants or biological fluids; validate in the relevant sample matrix.
• As a polyclonal antibody, lot‑to‑lot variability may exist; consider including appropriate controls and routine validation in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop IVD raw‑material sourcing partner, supplying validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target procurement. This anti‑mouse CX3CL1 rabbit polyclonal antibody supports chemokine and inflammation research, enabling seamless transition from early‑stage concept to clinical application development.