Anti-CWC22 Polyclonal Antibody for WB and ELISA - Q9HCG8

Core Product Specifications and Parameters

Biological Background: CWC22 Function and Localization

• CWC22 is a component of the spliceosome required for pre-mRNA splicing (PubMed:11991638, PubMed:12226669, PubMed:22961380). Related references: PMID:11991638 PMID:12226669 PMID:22961380
• It promotes exon-junction complex (EJC) assembly by hindering EIF4A3 from non-specifically binding RNA and escorting it to the splicing machinery (PubMed:22959432, PubMed:22961380). Related references: PMID:22959432 PMID:22961380
• As a component of the minor spliceosome, CWC22 is involved in splicing U12-type introns (Probable).
• Through its role in EJC assembly, CWC22 is required for nonsense-mediated mRNA decay.
• The protein localizes predominantly to the nucleus and nuclear speckles.
• CWC22 is subject to phosphorylation and is a reference proteome component (Phosphoprotein, Proteomics identification).
• The molecular weight of CWC22 is approximately 105 kDa (calculated from sequence: 105,466 Da).

Experimental Guidance and Technical Tips

• For Western blot, validate the antibody in your target cell lines; expect a band around 105 kDa.
• The immunogen (amino acids 639-908) shares high homology with mouse CWC22, supporting the observed cross-reactivity.
• When performing ELISA, consider optimizing the coating antigen and antibody dilution in your specific sample system.
• Always include appropriate positive and negative controls to confirm specificity in your experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a seamless sourcing channel for high-quality IVD raw materials like this CWC22 antibody. Our platform supports your splicing and mRNA metabolism studies with a comprehensive catalog of validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. For challenging targets or bulk requirements, our sourcing network delivers tailored solutions from concept to clinic.