Anti-CUX1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P39880 / Q13948

Core Product Specifications and Parameters

Biological Background: CUX1 Function and Localization

• CUX1 (Homeobox protein cut-like 1), also known as CDP (CCAAT displacement protein) and CDP/Cux p200, is a transcription factor with multiple isoforms generated through alternative splicing and proteolytic processing.
• It plays a central role in neuronal differentiation in the brain, regulating dendrite development, branching, and dendritic spine formation in cortical layers II–III, and also controls synaptogenesis.
• Acts as a transcriptional repressor by binding to CCAAT motifs, preventing positively acting CCAAT factors from binding; it is a component of the nf-munr repressor and binds to matrix attachment regions (MARs) of the immunoglobulin heavy chain enhancer.
• Represses T-cell receptor beta enhancer function by binding to MARbeta and may require TCF4 as a coactivator for other targets.
• CUX1 is involved in cell cycle progression at the G1/S transition; as cells enter S phase, proteolytic processing generates a stable, DNA-binding p110 fragment that activates genes like POLA1.
• Subcellular localization is primarily nuclear, consistent with its role as a transcription factor.
• The protein is subject to phosphorylation and ubiquitination (isopeptide bond formation), and mutations are linked to intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1–280 of human CUX1 (NP_001189472.1). Verify that your target protein includes this N-terminal region, especially if studying isoforms or processed forms.
• For western blot (WB), multiple bands around 72–77 kDa (p110 form) and 147–165 kDa (full-length p200) may be observed; optimization of sample preparation and electrophoresis conditions is recommended.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate antigen retrieval methods and antibody dilution for your specific tissue or cell type, as CUX1 expression patterns vary.
• The antibody shows cross-reactivity with mouse and rat CUX1; confirm performance in these species by parallel testing, particularly if using knockout controls.
• For ELISA applications, consider pairing with a detection antibody recognizing a different epitope; optimize coating and blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, providing validated polyclonal and monoclonal antibodies, antibody pairs, and ancillary reagents to streamline assay development. This CUX1 antibody supports research into transcriptional regulation, neuronal differentiation, and cell cycle processes. Leverage CamelBio’s supply network for bulk orders, rare target sourcing, and technical consultation to accelerate your transition from concept to clinic.