Anti-Cullin 4A Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q13619

Core Product Specifications and Parameters

Biological Background: Cullin-4A Function and Localization

• CUL4A serves as a core scaffold protein within cullin-RING E3 ubiquitin ligase complexes (CRLs), enabling ubiquitin transfer to substrate proteins by positioning both the substrate and the ubiquitin-conjugating enzyme. The ligase activity is dependent on cullin neddylation and is inhibited by association of deneddylated CUL4A with CAND1. Related references: PMID:14578910, PMID:14739464, PMID:15448697
• The DCX(DDB2) complex employs DDB2 as a substrate receptor to ubiquitinate XPC, histones H3-H4, and other targets, playing critical roles in nucleotide excision repair and replication-coupled nucleosome assembly. Related references: PMID:15811626, PMID:16678110, PMID:17041588, PMID:24209620
• Through the DCX(DTL) complex, CUL4A mediates PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of p53/TP53 in response to DNA damage and during DNA replication, and also directs autoubiquitination of DTL. Additionally, the DDB1-CUL4A-DTL complex regulates circadian rhythm by ubiquitinating CRY1 for degradation. Related references: PMID:14578910, PMID:15448697, PMID:15548678, PMID:23478445, PMID:26431207
• The DCX(ERCC8) complex (CSA complex) participates in transcription-coupled repair (TCR), removing DNA lesions from actively transcribed genes. Related references: PMID:12732143, PMID:32355176, PMID:38316879
• The DCX(AMBRA1) complex functions as a master regulator of the G1/S cell cycle transition by targeting phosphorylated cyclin D (CCND1, CCND2, CCND3) for ubiquitination and degradation. It also regulates CRL5 complexes through ubiquitination and degradation of Elongin-C (ELOC). Related references: PMID:33854232, PMID:33854239, PMID:30166453
• CUL4A is implicated in ubiquitination of numerous other substrates, including JUN (via DET1-COP1), CDKN1B/p27 (with DDB1-SKP2), and HOXA9, highlighting its broad regulatory roles. Related references: PMID:14739464, PMID:16537899, PMID:14609952
• Together with CUL4B, CUL4A contributes to ribosome biogenesis. Related references: PMID:26711351

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–300 of human CUL4A, indicating that the antibody targets the N-terminal region; this may influence epitope accessibility in certain assays.
• In Western blotting (WB), expect a band at approximately 88 kDa; include protease inhibitors during lysis and consider using fresh samples to avoid degradation.
• For immunoprecipitation (IP), validate the antibody’s ability to capture native CUL4A complexes under non-denaturing conditions.
• For immunofluorescence (IF/ICC), optimize fixation/permeabilization protocols, as CUL4A is predominantly nuclear; confirm staining specificity with appropriate controls.
• This antibody cross-reacts with human, mouse, and rat CUL4A; when using rodent samples, ensure that the detection system is compatible and that the target band is distinguishable from endogenous immunoglobulins in IP.
• As a polyclonal antibody, lot-to-lot variability may occur; consider titrating the antibody for each new application and including positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, enabling diagnostic manufacturers and research labs to accelerate development from concept to clinic. This anti-Cullin 4A polyclonal antibody is a valuable tool for investigating ubiquitin-mediated proteolysis, DNA damage repair, and cell cycle control, and CamelBio can further supply matched antibody pairs, recombinant proteins, and bulk ancillary reagents to support your complete workflow. For projects requiring rare target reagents, our team offers customized sourcing solutions to ensure you have the critical components needed.