Anti-Cullin-4A Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q13619

Core Product Specifications and Parameters

Biological Background: Cullin-4A Function and Localization

• CUL4A is the scaffold protein of cullin-RING E3 ubiquitin ligases, facilitating ubiquitin transfer by positioning substrate and E2 enzyme (PubMed:14578910, PubMed:14739464, PubMed:15448697). Related references: PMID:14578910 PMID:14739464 PMID:15448697

• E3 ligase activity depends on CUL4A neddylation; deneddylated CUL4A binds TIP120A/CAND1 to inhibit the complex (PubMed:14578910, PubMed:14739464, PubMed:15448697). Related references: PMID:14578910 PMID:14739464 PMID:15448697

• Substrate specificity is determined by DDB1-CUL4A-associated factors (DCAFs): DCX(DET1-COP1) ubiquitinates JUN (PubMed:14739464); DCX(DDB2) targets XPC (PubMed:15811626) and histones H3-H4 for nucleosome assembly (PubMed:16678110, PubMed:17041588, PubMed:24209620). Related references: PMID:14739464 PMID:15811626 PMID:16678110 PMID:17041588 PMID:24209620

• The DCX(DTL) complex polyubiquitinates CDT1 and p53/TP53 during DNA damage and replication (PubMed:14578910, PubMed:15448697, PubMed:15548678) and mediates DTL autoubiquitination (PubMed:23478445). Related references: PMID:14578910 PMID:15448697 PMID:15548678 PMID:23478445

• CUL4A, through DCX(ERCC8), is essential in transcription-coupled repair (TCR) (PubMed:12732143, PubMed:32355176, PubMed:38316879). Related references: PMID:12732143 PMID:32355176 PMID:38316879

• CUL4A participates in the DesCEND pathway by targeting proteins with C-terminal degrons via TRPC4AP- or DCAF12-containing DCX complexes (PubMed:29779948). Related references: PMID:29779948

• The DCX(AMBRA1) complex mediates G1/S transition by ubiquitinating cyclin D (PubMed:33854232, PubMed:33854239) and regulates CRL5 complexes by degrading Elongin-C (PubMed:30166453). Related references: PMID:33854232 PMID:33854239 PMID:30166453

• Together with CUL4B, CUL4A contributes to ribosome biogenesis (PubMed:26711351). Related references: PMID:26711351

• CUL4A is a phosphoprotein involved in host-virus interactions and is a key player in the ubiquitin-proteasome system, impacting DNA repair, cell cycle, and circadian rhythms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 660–759; therefore, the antibody likely recognizes a linear epitope in the C-terminal region of human CUL4A. This design often supports reliable detection in denaturing Western blot (WB) conditions.
• For WB, an expected band around 88 kDa should be observed; consider using a protein ladder with appropriate molecular weight markers and optimizing blocking and antibody incubation conditions for the specific sample lysate.
• For IHC-P, antigen retrieval methods (e.g., heat-induced epitope retrieval) may be beneficial; validate staining specificity in the relevant human tissue samples using appropriate controls.
• ELISA applications can benefit from optimizing coating antigen concentrations and detection systems to ensure linearity and sensitivity; the monoclonal nature of this antibody helps reduce lot-to-lot variability.
• As the antibody is raised in rabbit, use anti-rabbit IgG secondary antibodies for detection; cross-reactivity with other species has not been validated beyond human, so verify reactivity if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to IVD raw materials, supporting developers and manufacturers from concept to clinic. For research areas like ubiquitination, DNA repair, and cell cycle control, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whether you need this anti-Cullin-4A antibody or complementary reagents for E3 ligase studies, our B2B supply chain ensures quality and reliability.