Anti-Cullin 3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13618

Core Product Specifications and Parameters

Biological Background: Cullin-3 Function and Localization

• Cullin-3 (CUL-3) is a scaffold protein belonging to the cullin family and serves as the core component of multiple cullin‑RING‑based BCR (BTB‑CUL3‑RBX1) E3 ubiquitin‑protein ligase complexes. Together with ARIH1, these complexes mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Related references: PMID:29695787 PMID:27565346
• The ligase activity is dependent on neddylation of the cullin subunit and is inhibited by the association of the deneddylated subunit with CAND1/TIP120A.
• Substrate specificity is determined by the BTB‑domain‑containing adaptor protein. For example, the BCR(KEAP1) complex acts as a key sensor of oxidative stress by ubiquitinating NFE2L2/NRF2. Related references: PMID:15601839 PMID:16006525
• Other BCR complexes regulate diverse processes such as collagen export (BCR(KLHL12) via SEC31 monoubiquitination), ion transport in the kidney (BCR(KLHL3) targeting WNK4), and chromosomal passenger complex localization during mitosis (BCR(KLHL21) targeting AURKB). Related references: PMID:22358839 PMID:23387299 PMID:19995937
• Subcellular localization includes nucleus, cytoplasm, Golgi apparatus, centrosome, spindle, and cilia/flagella, consistent with its broad functional roles.
• Widely expressed, with relatively higher levels detected in brain, spermatozoa, and testis. Key post‑translational modifications include neddylation, acetylation, and phosphorylation. Sequence variants are associated with neurological disorders such as autism spectrum disorder and epilepsy.

Experimental Guidance and Technical Tips

• As a polyclonal antibody generated against a recombinant fragment (aa 569‑768), it recognizes multiple epitopes and is suitable for both denaturing (WB) and native (IF/ICC) conditions.
• For WB: expect a band around 89 kDa; consider running a dilution series to optimize signal-to‑noise in the relevant sample system.
• For IF/ICC: validate fixation and permeabilization protocols; the antibody may detect Cullin‑3 in various subcellular compartments (nucleus, cytoplasm, and centrosomes).
• For ELISA: the antibody can be used as either capture or detection reagent; pairing with a matched monoclonal antibody may improve assay specificity.
• Due to cross‑reactivity with mouse and rat, confirm endogenous expression levels in the specific cell line or tissue before quantitative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with a full spectrum of IVD raw materials, from validated antibody pairs and recombinant proteins to ancillary reagents. This anti-Cullin 3 antibody supports ubiquitin‑proteasome pathway studies, and CamelBio can also source related tools such as Cullin‑3 recombinant proteins, neddylation pathway inhibitors, or KEAP1/NRF2 assay kits. As a one‑stop B2B partner, we streamline procurement of rare‑target raw materials and custom‑development services to accelerate your project from concept to clinic.