Anti-Cullin 3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q13618

Core Product Specifications and Parameters

Biological Background: Cullin-3 Function and Localization

• CUL3 acts as a scaffold in Cullin-RING E3 ubiquitin ligase (CRL) complexes, mediating ubiquitination of target proteins for proteasomal degradation. Related references: PMID:29695787, PMID:27565346
• The functional specificity of each BCR complex is determined by the BTB domain-containing substrate recognition component. For instance, BCR(KLHL12) regulates COPII coat size in ER-Golgi transport, BCR(KLHL3) regulates ion transport via WNK4 ubiquitination, and BCR(KEAP1) senses oxidative stress by targeting NRF2 for degradation. Related references: PMID:22358839, PMID:27716508, PMID:23387299, PMID:23453970, PMID:23576762, PMID:15601839, PMID:16006525
• CUL3 is involved in cell cycle progression by targeting cyclin E and cyclin D1 for ubiquitination, thereby regulating G1/S transition.
• Subcellularly, CUL3 localizes to the nucleus, Golgi apparatus, centrosome, spindle poles, and cilia, reflecting its diverse roles in cell division, intracellular transport, and ciliogenesis.
• CUL3 is subject to neddylation, which activates the E3 ligase complex, and its activity is inhibited by CAND1 binding to the deneddylated form.
• CUL3 protects against human enterovirus D68 infection by mediating the ubiquitination and degradation of viral protein VP1. Related references: PMID:40396757
• Tissue expression: CUL3 is widely expressed, with higher levels detected in brain, spermatozoa, and testis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 468-768 within the C-terminal region of human Cullin-3, which may confer recognition across species due to sequence conservation.
• This polyclonal antibody is suitable for Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. Consider validating in the relevant sample system to confirm sensitivity and specificity.
• For WB, expect a band around 89 kDa. Use appropriate lysis and loading controls to verify target detection.
• When performing IF/ICC, optimize fixation and permeabilization conditions to preserve native protein localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For Cullin-3-related studies, we support your ubiquitin-proteasome and cell cycle research with reliable antibody reagents and can assist in sourcing rare targets or custom development needs. Let CamelBio be your trusted partner from concept to clinic.