Anti-Cullin 3 Monoclonal Antibody for WB, IHC-P, ELISA - Q13618

Core Product Specifications and Parameters

Biological Background: Cullin 3 Function and Localization

• Cullin-3 is the core scaffold of BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes, mediating ubiquitination of target proteins in cooperation with ARIH1. Related references: PMID:29695787 PMID:27565346

• Substrate specificity is conferred by BTB domain adaptors; for example, KEAP1 directs NRF2 ubiquitination in oxidative stress, and KLHL12 mediates SEC31 monoubiquitination for COPII coat regulation. Related references: PMID:15601839 PMID:16006525 PMID:22358839 PMID:27716508

• CUL3 localizes to multiple subcellular compartments including the nucleus, Golgi apparatus, centrosome, spindle, and cilium, reflecting its roles in mitotic regulation, ER-Golgi transport, and ciliogenesis.

• The ubiquitin ligase activity of CUL3 requires neddylation, while deneddylated CUL3 binds CAND1 to inhibit activity, providing a regulatory switch.

• BCR(KLHL22) regulates the TORC1 pathway by ubiquitinating DEPDC5 and enforces spindle assembly checkpoint silencing via PLK1 monoubiquitination. Related references: PMID:29769719 PMID:23455478

• CUL3 exhibits antiviral activity by mediating ubiquitination and degradation of human enterovirus D68 VP1. Related references: PMID:40396757

Experimental Guidance and Technical Tips

• For Western blot, an expected band at approximately 89 kDa can be observed; consider using brain or testis lysates as positive controls.
• For IHC-P, formalin-fixed paraffin-embedded sections of brain or testis are recommended, with appropriate antigen retrieval to enhance detection.
• In ELISA applications, validate the antibody’s performance with known concentrations of recombinant or endogenous Cullin 3 to assess sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive one-stop sourcing solution for IVD raw materials. For ubiquitination and cell cycle research, we offer validated Cullin 3 monoclonal antibodies alongside matched antibody pairs, bulk ancillary reagents, and rare-target raw-material sourcing. Our end-to-end service—from concept to clinic—streamlines your development workflow while keeping your target applications at the center.