Anti-Cullin 2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13617

Core Product Specifications and Parameters

Biological Background: Cullin 2 Function and Localization

• Cullin-2 (CUL2) is a core component of the ElonginB/C-CUL2/5-SOCS-box protein (ECS) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Related references: PMID:11384984 PMID:26138980 PMID:29775578
• Within the ECS complex, CUL2 serves as a rigid scaffold that positions both the substrate and the E2 ubiquitin-conjugating enzyme, contributing to catalytic efficiency. Related references: PMID:10973499 PMID:11384984 PMID:12609982
• The E3 ubiquitin ligase activity of the complex requires the neddylation of CUL2 and is inhibited upon binding of deneddylated CUL2 to TIP120A/CAND1. Related references: PMID:12609982 PMID:24076655 PMID:27565346
• ECS complexes containing the von Hippel-Lindau (VHL) substrate recognition subunit specifically ubiquitinate hypoxia-inducible factor (HIF), thereby regulating cellular responses to oxygen levels. Related references: PMID:10973499 PMID:9122164
• Several ECS complexes participate in the DesCEND pathway, which recognizes C-terminal degrons (C-degrons) on target proteins, leading to their ubiquitination and degradation. Related references: PMID:26138980 PMID:29775578 PMID:29779948
• CUL2-based ECS complexes cooperate with the E3 ligase ARIH1 in a tandem mechanism to mediate target protein ubiquitination. Related references: PMID:27565346
• Cullin-2 is predominantly localized in the nucleus, consistent with its role in nuclear protein turnover.
• The protein undergoes various post-translational modifications including acetylation and phosphorylation, and is subject to neddylation and other ubiquitin-like conjugations.

Experimental Guidance and Technical Tips

• The immunogen for this antibody corresponds to amino acids 40 – 310 of human Cullin 2 (NP_001185708.1), a region that may be conserved across isoforms. This design could allow detection of multiple CUL2 variants.
• For Western blot (WB), consider using reducing conditions and loading appropriate positive controls such as nuclear extracts from human cell lines (e.g., HeLa or HEK293). A band of approximately 87 kDa is expected.
• For immunofluorescence/immunocytochemistry (IF/ICC), validate fixation and permeabilization protocols; nuclear localization should be confirmed in human samples.
• In ELISA applications, pair this antibody with a compatible detection system; assay optimization may be needed depending on the target protein’s conformation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined procurement channel for IVD raw materials, serving as a single source for validated antibody pairs, polyclonal and monoclonal antibodies, and associated reagents. This high-quality anti-Cullin 2 polyclonal antibody supports research into ubiquitination pathways, cancer biology, and protein degradation mechanisms. For custom or bulk requirements, including rare target sourcing, our team offers consultative support to accelerate your assay development.