Anti-Cullin 2 (CUL2) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q13617

Core Product Specifications and Parameters

Biological Background: Cullin 2 Function and Localization

• Cullin-2 (CUL2) serves as a rigid scaffold protein in multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box) E3 ubiquitin ligase complexes, mediating substrate ubiquitination and subsequent proteasomal degradation. Related references: PMID:11384984, PMID:26138980, PMID:29775578

• The activity of the ECS complex is tightly regulated by cullin neddylation, which activates E3 ligase function, and by the inhibitor CAND1, which binds deneddylated CUL2 and prevents spurious substrate recruitment. Related references: PMID:12609982, PMID:24076655, PMID:27565346

• Among the substrate-specific ECS complexes, ECS(VHL) targets hypoxia-inducible factor (HIF) for ubiquitination, playing a critical role in cellular oxygen sensing and the hypoxic response. Related references: PMID:10973499, PMID:9122164

• Cullin-2 is also a key component of the DesCEND (destruction via C-end degrons) pathway, where substrate-recognition subunits such as KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B, or FEM1C recognize specific C-terminal motifs on target proteins, leading to their ubiquitin-dependent turnover. Related references: PMID:26138980, PMID:29775578, PMID:29779948

• ECS complexes and the ubiquitin ligase ARIH1 collaborate in a tandem mechanism to mediate efficient substrate ubiquitination. Related references: PMID:27565346

• CUL2 localizes predominantly to the nucleus, consistent with its role in regulating nuclear protein stability and transcription factor turnover.

• Post-translational modifications of Cullin-2 include neddylation, phosphorylation, and acetylation; alternative splicing generates multiple isoforms, and it participates in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human Cullin 2, an area likely to be exposed in both native and denatured conformations. Consider using this antibody in Western blotting under reducing conditions, with an expected band at 87–89 kDa.
• Due to cross-reactivity with mouse and rat, validate antibody specificity in these species by siRNA knockdown or knockout controls.
• For immunohistochemistry (paraffin sections), antigen retrieval using citrate buffer (pH 6.0) or EDTA buffer (pH 9.0) may enhance nuclear staining.
• Optimal ELISA performance depends on appropriate coating antigen and antibody titration; include a standard curve with recombinant CUL2 protein if available.
• Pairing this antibody with other Cullin family antibodies can help dissect specific E3 ligase complexes in immunoprecipitation applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive pipeline of IVD raw materials, connecting diagnostic developers with validated antibody pairs, high-quality recombinant antibodies, and bulk ancillary reagents. This Cullin 2 antibody supports research in ubiquitin-proteasome pathways, targeted protein degradation, and cancer biology. Rely on CamelBio for reliable sourcing and technical expertise from early-stage R&D through clinical application.