Anti-Cullin 1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13616

Core Product Specifications and Parameters

Biological Background: Cullin-1 Function and Localization

• Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. Related references: PMID:22017875, PMID:22017877, PMID:27565346
• In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Related references: PMID:38326650
• The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. Related references: PMID:12609982, PMID:38326650
• The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. For example, SCF(SKP2) directs ubiquitination of p27 to regulate G1/S transition, while SCF(BTRC) targets β-catenin in Wnt signaling.
• Expressed in lung fibroblasts.
• Subject to post-translational modifications including neddylation; involved in host-virus interactions and methylation pathways.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 435–593 (C-terminal region). In Western blotting, expect a band around 90 kDa; validate specificity using positive controls such as lung fibroblast lysates.
• For IHC-P, consider standard antigen retrieval protocols and include a known positive tissue (e.g., human lung section) to confirm staining.
• For IF/ICC, optimize fixation and permeabilization conditions; Cullin-1 localizes to both nucleus and cytoplasm.
• In ELISA, this antibody can be used for capture or detection; pair with a matched antibody set for optimal sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-Cullin-1 antibody is suitable for studying ubiquitin-proteasome pathways and cell cycle regulation. Beyond antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal reagents, and ancillary raw materials to streamline your assay development.