Anti-Cullin 1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q13616

Core Product Specifications and Parameters

Biological Background: Cullin 1 Function and Localization

• Cullin-1 (CUL1) is a core scaffold component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of key regulatory proteins involved in cell cycle progression, signal transduction, and transcription.
• SCF complexes, in collaboration with ARIH1, catalyze the ubiquitination of target proteins through a tandem mechanism that is essential for maintaining protein homeostasis and cellular signaling. Related references: PMID:22017875 PMID:22017877 PMID:27565346
• CUL1 functions as a rigid scaffold organizing SKP1-F-box protein and RBX1 subunits; full E3 ligase activity requires neddylation of CUL1, and substrate receptor exchange is mediated by CAND1. Related references: PMID:12609982 PMID:38326650
• Specific SCF complexes recognize distinct substrates: SCF(BTRC) and SCF(FBXW11) direct the ubiquitination of CTNNB1, NFKBIA, NFKBIB, and CEP68, thereby regulating Wnt and NF-κB signaling pathways. Related references: PMID:25503564 PMID:25704143
• Neddylated CUL1-RBX1 complex ubiquitinates p53/TP53 when recruited by the Cul7-RING(FBXW8) complex, linking CUL1 to tumor suppressor regulation. Related references: PMID:35982156
• In T-cell receptor signaling, SCF(BTRC) mediates 'Lys-48'-linked ubiquitination of UBR2, highlighting CUL1's role in immune modulation. Related references: PMID:38225265
• Under oxidative stress, the SCF(FBXO31) complex ubiquitinates proteins with C-terminal amidation, expanding CUL1's functional repertoire. Related references: PMID:39880951
• CUL1 is expressed in lung fibroblasts and is subject to post-translational modifications including methylation, neddylation, and isopeptide bond formation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 435–593 of human Cullin 1 (NP_003583.2), suggesting that the antibody recognizes an epitope within the C-terminal region of the protein.
• For Western blotting, a specific band at approximately 90 kDa is expected in lysates from human, mouse, and rat cells or tissues.
• In immunohistochemistry (IHC-P), consider optimizing antigen retrieval and antibody dilution, as the recognition of native protein may require specific conditions.
• This polyclonal antibody may also be suitable for ELISA-based assays; ensure proper coating and detection conditions are validated for each new application.
• Given the broad cross-reactivity with human, mouse, and rat, it is advisable to verify specificity in the relevant species and tissue type before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing platform for IVD raw materials, offering diagnostic manufacturers and research laboratories access to validated antibody pairs, bulk ancillary reagents, and rare target sourcing. For your Cullin 1 research in ubiquitination and cell cycle pathways, we provide high-quality polyclonal antibodies and complementary reagents to accelerate your assay development and clinical translation. Let us streamline your procurement from discovery to diagnostic application.