Anti-CUL5 Polyclonal Antibody for WB, ELISA - Q93034

Core Product Specifications and Parameters

Biological Background: CUL5 Function and Localization

• CUL5 encodes the scaffold protein cullin-5 (VACM-1) that forms the core of multiple cullin-5-RING E3 ubiquitin ligase complexes (CRL5), positioning substrates and ubiquitin-conjugating enzymes for ubiquitination and proteasomal degradation. Related references: PMID:11384984, PMID:15601820, PMID:21199876, PMID:33268465

• The substrate specificity of CRL5 is conferred by SOCS box proteins; for instance, ECS(SOCS1) ubiquitinates JAK2, while ECS(SOCS2) targets phosphorylated EPOR and GHR. Related references: PMID:11384984, PMID:21980433, PMID:25505247

• CUL5 regulates neuron positioning during cortex development by mediating DAB1 degradation via SOCS7-containing ECS complexes (By similarity).

• CRL5 complexes control cell migration, actin dynamics, and chromatin modification by targeting proteins such as ANKRD28 (via RAB40C), RHOU (via RAB40A), LIMA1 (via RAB40B), and SUV39H1 (via ASB7). Related references: PMID:35512830, PMID:26598620, PMID:33999101, PMID:40440427

• During HIV-1 infection, viral Vif protein redirects CUL5 to form an E3 ligase that ubiquitinates and degrades host antiviral factors APOBEC3F, APOBEC3G, and APOBEC3H. Related references: PMID:16636053, PMID:22190037, PMID:37640699

• CUL5 is predominantly nuclear and subject to phosphorylation and isopeptide bond formation.

• The predicted molecular weight of CUL5 is ~91 kDa.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein corresponding to the N-terminal region (aa 1-300) of human CUL5
• For Western blotting, consider using whole‑cell lysates from human, mouse, or rat cells; expect to detect a band at ~91 kDa
• For ELISA applications, validate in the relevant sample system and optimize coating/blocking conditions empirically
• Pre-adsorption or blocking with the recombinant immunogen may improve specificity in certain assays

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, supporting research in ubiquitination, proteasomal degradation, and antiviral immunity. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and efficient rare-target raw-material procurement to accelerate your diagnostic development from concept to clinic.