Anti-CTU2 Polyclonal Antibody for WB, ELISA - Q2VPK5

Core Product Specifications and Parameters

Biological Background: CTU2 Function and Localization

• CTU2, also known as C16orf84, NCS2, MFRG, or UPF0432, is the cytoplasmic tRNA 2-thiolation protein 2, a member of the NCS2/CTU2 family.
• It plays a central role in the 2-thiolation of mcm5S2U at wobble positions of tRNALys, tRNAGlu, and tRNAGln, which is critical for accurate and efficient translation.
• CTU2 functions as part of a heterodimer with CTU1 (ATPBD3), catalyzing the ATP-dependent transfer of sulfur from thiocarboxylated URM1 to uridine residues in tRNAs.
• Subcellular localization studies indicate CTU2 is predominantly cytoplasmic, consistent with its role in cytosolic tRNA modification.
• Post-translational modifications of CTU2 include acetylation and phosphorylation, which may regulate its activity and interactions.
• Alternative splicing of the CTU2 gene produces multiple isoforms, potentially contributing to functional diversity.
• Mutations in CTU2 have been linked to primary microcephaly, highlighting its importance in neurological development.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region of CTU2 (amino acids 186–515). When performing Western blot, the detected band is expected around 56 kDa; consider using appropriate molecular weight markers.
• For ELISA applications, validate the antibody with the specific immunogen or recombinant protein to ensure optimal binding.
• Since CTU2 is conserved in human and rat, the antibody is suitable for cross-species studies; however, we recommend verifying cross-reactivity in your specific experimental system.
• Consider testing in relevant cell lines or tissue extracts where CTU2 is expressed, such as those with high tRNA modification activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop provider of IVD raw materials, supporting diagnostic developers and research laboratories with a comprehensive portfolio of antibodies, reagents, and custom sourcing solutions. Our anti-CTU2 polyclonal antibody is part of a broad catalog of rare target antibodies, complemented by validated antibody pairs and bulk ancillary reagents to accelerate your assay development and manufacturing needs. Whether you require specialized raw materials for tRNA modification research or scalable supply for diagnostic kits, CamelBio bridges the gap from concept to clinic.