Anti-CTSV (Cathepsin L2) Rabbit Polyclonal Antibody for WB and ELISA - O60911

Core Product Specifications and Parameters

Biological Background: CTSV (Cathepsin L2) Function and Localization

• CTSV, also known as cathepsin L2, cathepsin U, or cathepsin V, is a lysosomal cysteine protease encoded by the CTSV gene in humans.
• It functions as a thiol protease, likely involved in protein degradation within lysosomes, and may have a particular role in corneal physiology.
• The enzyme is synthesized as a zymogen and undergoes proteolytic processing to generate the active form; it contains disulfide bonds and is glycosylated.
• Subcellular localization is primarily lysosomal, consistent with its role in intracellular proteolysis.
• Tissue expression is predominantly in the thymus and testis, with significant expression also in the corneal epithelium; lower levels are found in conjunctival epithelium and skin.
• As a member of the papain family of cysteine proteases, CTSV shares structural and functional similarities with cathepsin L, but exhibits distinct substrate specificity and tissue distribution.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 105-334 of human CTSV, which includes the mature catalytic region; thus, the antibody may recognize both pro and mature forms.
• For Western blot analysis, consider reducing conditions to account for intramolecular disulfide bonds; the predicted molecular weight is ~37 kDa, but glycosylation may cause slight shifts.
• Validation in endogenous systems may include human thymus, testis, or corneal epithelium lysates, as these tissues show high CTSV expression.
• In ELISA, the antibody may be used for capture or detection; however, optimal dilutions and pairings should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibodies, optimized reagents, and rare-target sourcing from discovery to commercialization. This CTSV polyclonal antibody is part of our protease and corneal research portfolio, supported by our capability to supply bulk ancillary reagents and customized antibody pairs. We bridge the gap between research and diagnostic manufacturing, ensuring robust supply chains for your catheterization and immunoassay development needs.