Anti-CTSS Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P25774

Core Product Specifications and Parameters

Biological Background: Cathepsin S Function and Localization

• Cathepsin S (CTSS) is a lysosomal thiol protease belonging to the papain family, with a molecular weight of approximately 37 kDa.
• It plays a critical role in MHC class II antigen presentation by removing the invariant chain chaperone, a step essential for peptide loading. Related references: PMID:30612035
• The bond specificity of cathepsin S partially overlaps with that of cathepsin L, suggesting complementary proteolytic functions. Related references: PMID:30612035
• Beyond immunity, cathepsin S elicits itch by cleaving and activating the Mas-related G protein-coupled receptor X1 (MRGPRX1).
• Subcellularly, it is primarily localized in lysosomes and is also found in cytoplasmic vesicles, phagosomes, and secreted extracellularly.
• Key structural features include alternative splicing, disulfide bonds, glycosylation, and a signal peptide; it is synthesized as an inactive zymogen.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature enzyme region (aa 115-331), likely detecting both pro‑ and active forms. Verify band pattern in your sample system.
• For Western blot, expect a band near 37 kDa under reducing conditions. Optimize loading and antibody dilution as needed.
• In IF/ICC, anticipate lysosomal/vesicular staining; co‑staining with organelle markers may aid confirmation.
• This polyclonal antibody reacts with human, mouse, and rat CTSS, but cross‑reactivity should be independently validated in each species.
• For ELISA, pair with appropriate detection reagents and validate against recombinant protein or relevant biological matrices.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti‑CTSS polyclonal antibody supports protease and immunology research in WB, IF/ICC, and ELISA formats. Beyond this product, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing to accelerate your diagnostic development.