Anti-CTSH Rabbit Polyclonal Antibody for WB, ELISA - P09668

Core Product Specifications and Parameters

Biological Background: Pro-cathepsin H Function and Localization

• Pro-cathepsin H (CTSH), also known as cathepsin H, is a lysosomal cysteine protease of the papain family, critically involved in the overall degradation of proteins within lysosomes.
• The enzyme is synthesized as an inactive zymogen (pro-cathepsin H) and undergoes proteolytic cleavage to remove the propeptide, generating the mature, active thiol protease.
• Cathepsin H is localized primarily in lysosomes, consistent with its role in intracellular protein turnover and homeostasis.
• It is a glycoprotein with intramolecular disulfide bonds that contribute to its structural stability and proper folding.
• As a member of the lysosomal hydrolase family, cathepsin H participates in normal cellular protein catabolism and may have additional roles in antigen processing and tissue remodeling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 23–140 of human pro-cathepsin H (NP_004381.2), covering part of the propeptide region. For Western blot, use reducing conditions and consider that the antibody may detect the full-length proenzyme (~37 kDa) as well as any processed intermediates, depending on the sample's lysosomal activation state.
• Validate the antibody in your specific sample system (e.g., lysates from human, mouse, or rat cells or tissues) to confirm reactivity and specificity under your experimental conditions.
• For ELISA applications, this polyclonal antibody may be used as either a capture or detection reagent. Ensure proper titration and evaluate pairing with a compatible secondary or detection antibody for optimal performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a one-stop sourcing solution for IVD raw materials, from concept to clinic. Our validated anti-CTSH rabbit polyclonal antibody supports research into lysosomal proteolysis and protease biology, and we can further supply matched antibody pairs, recombinant proteins, and bulk ancillary reagents. Leverage our expertise to streamline your procurement of rare-target raw materials and accelerate your diagnostic development.