Anti-CTSF Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UBX1

Core Product Specifications and Parameters

Biological Background: Cathepsin F Function and Localization

• Cathepsin F (CTSF) is a lysosomal cysteine protease of the papain family, encoded by the CTSF gene.
• Functions as a thiol protease involved in intracellular protein degradation and turnover within lysosomes.
• Implicated in tumor invasion and metastasis, potentially through extracellular matrix remodeling.
• Subcellular location: primarily lysosomal, consistent with its role in terminal protein degradation.
• Tissue specificity: high expression in heart, skeletal muscle, brain, testis, and ovary; moderate in prostate, placenta, liver, colon; absent in peripheral leukocytes and thymus.
• Post-translational modifications: synthesized as an inactive zymogen, activated by proteolytic cleavage; contains disulfide bonds and N-linked glycosylation sites.
• Associated with disease: mutations in CTSF are linked to neuronal ceroid lipofuscinosis (CLN13), a neurodegenerative disorder.
• Keywords: glycoprotein, hydrolase, lysosome, neurodegeneration, thiol protease, zymogen.

Experimental Guidance and Technical Tips

• For Western blot, consider using reducing conditions and a molecular weight marker to identify bands around 53 kDa (precursor) and potentially smaller active forms.
• For immunohistochemistry, antigen retrieval with citrate buffer may enhance staining due to lysosomal localization.
• In ELISA, the antibody may be suitable for detecting recombinant or native CTSF; optimize coating and blocking conditions.
• Confirm specificity with appropriate controls, such as peptide blocking or knockdown cell lines, especially when analyzing tissues with high endogenous CTSF expression.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive supplier for IVD manufacturers and research teams, CamelBio provides high-quality antibody raw materials, including this anti-CTSF polyclonal antibody, supporting studies on lysosomal proteases and cancer biology. We also offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your diagnostic development.