Anti-CTNNBIP1 Rabbit Monoclonal Antibody for WB and ELISA - Q9NSA3

Core Product Specifications and Parameters

Biological Background: CTNNBIP1 Function and Localization

• CTNNBIP1 (Beta-catenin-interacting protein 1), also known as ICAT (Inhibitor of beta-catenin and Tcf-4), is a negative regulator of the canonical Wnt signaling pathway.
• The protein functions by binding to beta-catenin and preventing its interaction with TCF/LEF transcription factors, thereby inhibiting Wnt target gene expression.
• Subcellular localization studies indicate that CTNNBIP1 resides in both the cytoplasm and nucleus, consistent with its role in modulating beta-catenin nuclear translocation and activity.
• CTNNBIP1 is classified as a phosphoprotein, suggesting that its activity may be regulated by phosphorylation.
• The protein has a molecular weight of approximately 9.2 kDa (UniProt) and is a reference proteome entry.
• A three-dimensional structure has been solved, providing insights into its inhibitory mechanism.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-81); for ELISA, the immunizing peptide can serve as a positive control.
• Due to its small size (~9 kDa), use a low molecular weight cutoff membrane (e.g., PVDF with 0.2 µm pore size) and optimize transfer conditions for Western blotting to prevent loss.
• Consider testing the antibody in human cell lines with active Wnt signaling (e.g., HEK293, HeLa) and include a negative control with silenced CTNNBIP1 expression to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, including validated monoclonal antibodies like this anti-CTNNBIP1 clone for Wnt pathway research. We supply bulk antibody pairs, optimized recombinant antibodies, and ancillary reagents to support diagnostic assay development and production. For specialized needs, our team can assist with rare target sourcing and peptide immunogen design.