Anti-CTNNA3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UI47

Core Product Specifications and Parameters

Biological Background: Catenin alpha-3 Function and Localization

• Catenin alpha-3 (CTNNA3), also known as alpha T-catenin or cadherin-associated protein, is a member of the alpha-catenin family involved in cell-cell adhesion.
• It may play a role in the formation of stretch-resistant cell-cell adhesion complexes, contributing to mechanical integrity of tissues.
• CTNNA3 is localized to the cytoplasm, cytoskeleton, and cell junctions, particularly desmosomes, where it links cadherin complexes to the actin cytoskeleton.
• Predominantly expressed in heart and testis, with lower expression in brain, kidney, liver, and skeletal muscle, suggesting tissue-specific adhesive functions.
• The protein undergoes alternative splicing and phosphorylation, and is associated with cardiomyopathy and disease variants, highlighting its importance in cardiac function.
• UniProt Keywords: Alternative splicing, Cardiomyopathy, Cell adhesion, Cell junction, Coiled coil, Cytoplasm, Cytoskeleton, Disease variant, Phosphoprotein, Proteomics identification, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-240) of human CTNNA3, which may help in detecting full-length protein and specific isoforms; consider epitope exposure in different sample preparations.
• For Western blotting (WB), a band around 100kDa is expected; validate with appropriate positive controls such as heart or testis lysates, and optimize blocking and antibody dilution.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), verify staining patterns in tissue sections or cells known to express CTNNA3, using antigen retrieval and appropriate fixation; expect localization at cell junctions and desmosomes.
• When using ELISA, consider using the recombinant immunogen as a positive control to establish sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers focusing on cell adhesion and cardiomyopathy, we offer sourcing support for CTNNA3 antibodies and related cadherin-catenin complex reagents, streamlining your development from concept to clinic.