Anti-CTNNA3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9UI47

Core Product Specifications and Parameters

Biological Background: Catenin alpha-3 Function and Localization

• CTNNA3 encodes catenin alpha-3, also known as alpha T-catenin, a cadherin-associated protein that plays a role in cell-cell adhesion.
• The protein may be involved in the formation of stretch-resistant adhesion complexes, linking cadherins to the actin cytoskeleton.
• It localizes predominantly to the cytoplasm, cytoskeleton, and desmosomes at cell junctions, supporting mechanical integrity in tissues.
• Expression is highest in heart and testis, with lower levels detected in brain, kidney, liver, and skeletal muscle, suggesting tissue-specific adhesive functions.
• The gene undergoes alternative splicing, producing multiple isoforms that may have distinct roles in adhesion and signaling.
• Mutations in CTNNA3 are associated with cardiomyopathy (e.g., arrhythmogenic right ventricular dysplasia), highlighting its importance in cardiac muscle cell cohesion.
• Post‑translational modifications include phosphorylation, which may regulate its activity and interactions at cell junctions.
• The protein contains coiled-coil domains and belongs to the vinculin/alpha-catenin superfamily, featuring conserved regions for binding actin and cadherin–catenin complexes.

Experimental Guidance and Technical Tips

• For Western blot, a band at approximately 100 kDa (predicted molecular weight ~99.8 kDa) is expected. Verify specificity by using appropriate positive and negative control lysates.
• In immunohistochemistry (paraffin), optimize antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) and validate staining in human, mouse, or rat tissues known to express CTNNA3, such as heart.
• For ELISA, the antibody can be explored as a capture or detection reagent; however, end‑users should confirm linearity and sensitivity with recombinant protein or native samples.
• Because the immunogen spans amino acids 700–895, this monoclonal antibody likely recognizes multiple isoforms containing this region. Researchers should assess isoform specificity if needed.
• Cross‑reactivity with human, mouse, and rat CTNNA3 has been demonstrated. Additional species may require experimental verification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw‑material procurement by offering validated monoclonal antibodies like this CTNNA3 clone, along with matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Researchers and diagnostic developers working on cell adhesion, cardiomyopathy, or desmosome biology can rely on CamelBio’s integrated supply chain to advance projects from concept to clinic efficiently.