Anti-CTIP2/BCL11B Monoclonal Antibody for WB, IHC-P, ELISA - Q9C0K0

Core Product Specifications and Parameters

Biological Background: CTIP2/BCL11B Function and Localization

• CTIP2/BCL11B is a critical transcription factor for T-cell lineage commitment and thymocyte development, modulating the expression of chemokine receptors CCR7 and CCR9 to direct progenitor migration from bone marrow to thymus. Related references: PMID:27959755
• Functions as a transcriptional repressor and tumor-suppressor by directly binding GC-rich response elements to silence target genes; may also participate in the p53 signaling pathway.
• Enhances IL2 expression in activated CD4+ T-lymphocytes, contributing to immune response regulation. Related references: PMID:16809611
• Localizes predominantly to the nucleus, consistent with its role as a transcription factor, and is highly expressed in brain and malignant T-cell lines from adult T-cell leukemia/lymphoma patients.
• The protein contains multiple zinc-finger domains and undergoes post-translational modifications including acetylation, methylation, and phosphorylation, influencing its regulatory functions.
• Alternative splicing produces isoforms such as ATL1-alpha, ATL1-beta, ATL1-gamma, and ATL1-delta, which may have distinct biological activities.

Experimental Guidance and Technical Tips

• For Western blot, a band around 96 kDa is expected; consider using whole-cell lysates from T-cell lines or tissues and validate with appropriate positive and negative controls.
• In IHC-P, nuclear staining is anticipated; optimize antigen retrieval conditions and antibody dilution on known positive control tissues (e.g., thymus or lymphoma specimens).
• ELISA applications may involve coating the antibody or using it as a detection reagent; pair with a suitable capture antibody if developing a sandwich assay and validate in the relevant sample matrix.
• Since cross-reactivity is confirmed for human and mouse, testing on both species’ samples is recommended to verify performance in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, from validated monoclonal antibodies like this anti-CTIP2/BCL11B antibody to bulk ancillary reagents and rare target development. Whether you are building assays for T-cell leukemia or immunology research, our portfolio supports every stage from concept to clinic, ensuring reliable access to critical components for diagnostic manufacturers and laboratories worldwide.