Anti-CTIP1/BCL11A Monoclonal Antibody for WB, IHC-P, ELISA - Q9H165

Core Product Specifications and Parameters

Biological Background: CTIP1/BCL11A Function and Localization

• CTIP1/BCL11A is a transcription factor that associates with the BAF SWI/SNF chromatin remodeling complex to regulate gene expression. Related references: PMID:23644491 PMID:39607926
• It binds the 5'-TGACCA-3' motif in regulatory regions, including the HBG1 distal promoter, directly repressing fetal hemoglobin and controlling the gamma- to beta-globin developmental switch. Related references: PMID:29606353 PMID:39423807 PMID:26375765
• Involved in brain development, with expression in both fetal and adult brain. Related references: PMID:27453576
• Plays a role in hematopoiesis and is essential for B-cell formation in fetal liver.
• Functions as a modulator of the transcriptional repression activity of NR2F2.
• Subcellularly localizes to the cytoplasm, nucleus, chromosome, and nuclear matrix.
• Expressed at high levels in brain, spleen, thymus, bone marrow, testis, and in CD34+ myeloid precursor cells, B-cells, monocytes, and megakaryocytes; tightly regulated during B-cell development.
• Features zinc-finger DNA-binding domains, metal-binding sites, phosphorylation, isopeptide bond formation, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 401-589 of human CTIP1/BCL11A; consider this region when designing epitope-specific detection strategies.
• For Western blotting, a band near 91 kDa is expected; validate specificity using knockout (KO) cell lines if available.
• IHC-P applications may require antigen retrieval optimization; confirm staining patterns in relevant human or rat tissue sections.
• ELISA protocols should be validated with recombinant protein or cell lysates; the antibody may be suitable for capture or detection roles in sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a one-stop access to IVD raw materials. For transcription regulation and hematopoiesis research like CTIP1/BCL11A studies, we supply validated monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing. Our offerings include matched antibody pairs and optimized clones to streamline your assay development from concept to clinic.