Anti-CTGF Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P29279

Core Product Specifications and Parameters

Biological Background: CTGF Function and Localization

• CCN family member 2, also known as connective tissue growth factor, hypertrophic chondrocyte-specific protein 24 (HCS24), and insulin-like growth factor-binding protein 8 (IGFBP-8).
• Major connective tissue mitoattractant secreted by vascular endothelial cells; promotes proliferation and differentiation of chondrocytes.
• Plays a critical role in osteogenesis by stimulating osteoblast differentiation (PubMed:39414788). Related references: PMID:39414788
• Mediates heparin- and divalent cation-dependent cell adhesion in various cell types including fibroblasts, myofibroblasts, endothelial, and epithelial cells.
• Enhances fibroblast growth factor-induced DNA synthesis.
• Localized to the extracellular space and secreted into the extracellular matrix.
• Expressed in bone marrow, thymic cells, and certain Wilms tumors; alternative splicing generates multiple isoforms.
• Glycoprotein with disulfide bonds; heparin-binding activity; associated with disease variants such as dwarfism.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 100–200) is conserved across human, mouse, and rat CTGF, supporting the reported cross-reactivity; sequence alignment may be verified for additional species.
• For Western blot, detect a band at approximately 38 kDa in lysates from relevant tissues or cell lines; validate expression in the sample system of interest.
• In IHC-P, antigen retrieval and endogenous peroxidase blocking are recommended; test with positive control tissues (e.g., bone marrow) and optimize primary antibody dilution.
• For IF/ICC, fixation and permeabilization conditions should be optimized; a secreted target may require inhibition of protein secretion for cellular staining.
• This polyclonal antibody may be suitable for ELISA as either capture or detection reagent; pairwise testing with appropriate standards is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and clinical institutions with streamlined access to high-quality IVD raw materials, technical support, and consulting—from initial concept through clinical application. This CTGF polyclonal antibody serves as a reliable reagent for connective tissue biology and musculoskeletal disease research. Our comprehensive supply capabilities include validated antibody pairs, optimized mono- and polyclonal antibodies, bulk ancillary reagents, and flexible sourcing for rare or customized targets to accelerate your development workflow.