Anti-CTGF Polyclonal Antibody for WB, IF/ICC, ELISA - P29279

Core Product Specifications and Parameters

Biological Background: CTGF Function and Localization

• CTGF (Connective Tissue Growth Factor), also known as CCN2, is a member of the CCN family of matricellular proteins; alternative names include NOV2, HCS24, and IGFBP8 (IGF-binding protein 8).
• It functions as a major mitoattractant secreted by vascular endothelial cells, promoting proliferation and differentiation of chondrocytes and playing a critical role in osteogenesis. Related references: PMID:39414788
• Mediates heparin- and divalent cation-dependent cell adhesion in fibroblasts, myofibroblasts, endothelial, and epithelial cells.
• Enhances fibroblast growth factor-induced DNA synthesis.
• Localizes to the extracellular matrix and is secreted into the extracellular space.
• Expressed in bone marrow, thymic cells, and some Wilms tumors.
• Contains disulfide bonds and is a heparin-binding glycoprotein, reflecting its extracellular matrix association and role in connective tissue remodeling.
• Disease variants are associated with dwarfism.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 100–200 of human CTGF; the antibody may recognize both secreted and intracellular forms. For WB, a band of approximately 38 kDa is expected—validate in your specific sample matrix with appropriate loading controls.
• For immunofluorescence (IF/ICC, IF-P), consider permeabilization to detect intracellular CTGF; secreted protein may also be found associated with the cell surface or extracellular matrix.
• Given the secreted nature of CTGF, avoid cross-linking fixatives like formaldehyde for IHC/IF if extracellular staining is desired, as crosslinking may reduce accessibility.
• Cross-reactivity with rat has been reported; verify performance in your rat tissue/cell system by running appropriate controls.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; consider revalidating critical assays with each new batch.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material partner, CamelBio supports your CTGF-related diagnostic and research projects with a curated selection of validated antibodies, including this polyclonal antibody for multiple applications. Beyond single reagents, we offer optimized monoclonal/polyclonal pairs, bulk ancillary reagents, and sourcing of rare targets to streamline your workflow from concept to clinic.