Anti-CTCFL Rabbit Polyclonal Antibody for WB and ELISA - Q8NI51

Core Product Specifications and Parameters

Biological Background: CTCFL Function and Localization

• CTCFL, also known as BORIS (Brother of the Regulator of Imprinted Sites) or CT27, is a testis-specific transcriptional repressor and paralog of the ubiquitously expressed CTCF protein.
• It functions as a DNA-binding protein that regulates insulator activity, nuclear architecture, and transcription, in part by recruiting epigenetic modifiers.
• Critical for male germline development, CTCFL participates in imprinting control at the IGF2/H19 locus by binding unmethylated H19 ICR and recruiting PRMT7 to deposit H4R3sme2 marks.
• Acts as a tumor suppressor and may be involved in activating BAG1 expression through interactions with DNMT1 and DNMT3B.
• Subcellularly, CTCFL is found in both the cytoplasm and nucleus.
• Expression is restricted to the testis, specifically in primary spermatocytes.
• Contains multiple zinc finger domains and is classified under keywords: activator, chromatin regulator, alternative splicing, and cell cycle-related functions.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-270 of human CTCFL, encompassing the N-terminal region which shows low homology to CTCF, potentially enhancing paralog specificity.
• For Western blot, the predicted molecular weight is 76 kDa; include appropriate positive (e.g., testis lysate) and negative controls, and validate band size carefully.
• In ELISA, ensure the coating antigen preserves the native epitope structure for optimal antibody recognition; consider testing direct vs. indirect formats.
• Cross-reactivity with mouse and rat is reported, but sequence conservation should be verified if working with these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, covering every stage from concept to clinic. This anti-CTCFL polyclonal antibody supports research into transcriptional regulation and cancer-testis antigens, and is part of our extensive portfolio of validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. Need a rare target or custom raw material? We help diagnostic manufacturers and labs secure reliable supplies efficiently.