Anti-CTCF Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA, ChIP, ChIP-seq, CUT&Tag - P49711

Core Product Specifications and Parameters

Biological Background: CTCF Function and Localization

• CTCF, also known as CCCTC-binding factor or 11-zinc finger protein, is a chromatin binding factor that binds to sequence-specific DNA sites and regulates the 3D structure of chromatin by forming loops and anchoring DNA to nuclear structures. Related references: PMID:16949368, PMID:18347100, PMID:18654629
• It defines boundaries between active and heterochromatic DNA by binding to chromatin insulators, preventing interactions between promoters and nearby enhancers and silencers, thereby mediating long-range gene silencing. Related references: PMID:18347100, PMID:18654629, PMID:19322193
• CTCF participates in allele-specific gene expression at the imprinted IGF2/H19 locus, mediating higher-order chromatin conformation and restricting enhancer access to IGF2 on the maternal allele. Related references: PMID:16107875, PMID:16815976, PMID:17827499
• It plays a critical role in X-chromosome inactivation by establishing a regulatable epigenetic switch and preventing the propagation of stable methylation at escape genes. Related references: PMID:11743158
• CTCF is localized primarily in the nucleus and associates with centromeres and chromosomal arms during metaphase, where it is required for cohesin localization and sister chromatid cohesion. Related references: PMID:18550811, PMID:26321640
• It acts as a transcriptional repressor of MYC and BAG1 and as a transcriptional activator of APP, demonstrating its dual regulatory functions. Related references: PMID:8649389, PMID:9591631, PMID:9407128
• The protein contains multiple post-translational modification sites, including acetylation, phosphorylation, and sumoylation (isopeptide bond), which regulate its chromatin-binding and transcriptional activities.
• CTCF is ubiquitously expressed in human tissues but is notably absent in primary spermatocytes, indicating tissue-specific regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 628–727 in the C-terminal region of human CTCF, a domain distinct from the zinc finger DNA-binding region, potentially enhancing antibody specificity for endogenous CTCF over other zinc finger proteins.
• For Western blotting, an expected band at approximately 83 kDa is predicted; actual migration may differ due to post-translational modifications or gel conditions, so validate with a known positive control.
• For chromatin immunoprecipitation (ChIP) and ChIP-seq, optimize crosslinking and chromatin shearing conditions for your cell type; include positive (e.g., H19 ICR) and negative control genomic regions to assess enrichment.
• When performing immunofluorescence, fixation and permeabilization protocols should be empirically tested to ensure robust nuclear staining; subcellular localization to the nucleus and centromeres may be observed.
• The antibody is suitable for CUT&Tag, offering an efficient alternative to ChIP-seq with lower cell input; validation of enzyme-tagging conditions and antibody concentration is recommended for optimal results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive IVD raw-material sourcing partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline your diagnostic development. For chromatin biology and gene regulation research, our CTCF antibody supports diverse applications from basic science to clinical assay design. Contact CamelBio for custom antibody sourcing, technical consultation, or scalable supply solutions.