Anti-CTCF Polyclonal Antibody for WB, IP, IF/ICC, IHC-P, ChIP, ELISA - P49711

Core Product Specifications and Parameters

Biological Background: CTCF Function and Localization

• CTCF is a highly conserved zinc-finger DNA-binding protein that functions as a key architectural regulator of chromatin organization and gene expression (PubMed:16949368, PubMed:18347100, PubMed:18654629). Related references: PMID:16949368 PMID:18347100 PMID:18654629
• It mediates chromatin loop formation, anchors DNA to the nuclear lamina, and defines boundaries between active and heterochromatic domains by binding to chromatin insulators (PubMed:18347100, PubMed:18654629, PubMed:19322193). Related references: PMID:18347100 PMID:18654629 PMID:19322193
• CTCF participates in allele-specific gene expression at the imprinted IGF2/H19 gene locus and regulates asynchronous replication of this locus (PubMed:16107875, PubMed:16815976, PubMed:17827499). Related references: PMID:16107875 PMID:16815976 PMID:17827499
• It preferentially interacts with unmethylated DNA, preventing the spread of CpG methylation; its binding is inhibited by CpG methylation, thus linking DNA methylation to chromatin structure (PubMed:18413740). Related references: PMID:18413740
• Acts as a transcriptional repressor at the MYC and BAG1 promoters, and as an activator of APP; also regulates the APOA1/C3/A4/A5 gene cluster and MHC class II genes (PubMed:8649389, PubMed:9591631, PubMed:9407128, PubMed:18347100, PubMed:19322193). Related references: PMID:8649389 PMID:9591631 PMID:9407128 PMID:18347100 PMID:19322193
• CTCF can dimerize, causing local histone modification changes (loss of acetylation, gain of methylation) and is required for nucleosome positioning, sister chromatid cohesion, and cohesin localization (PubMed:12191639, PubMed:18550811). Related references: PMID:12191639 PMID:18550811
• Localizes to the nucleus, nucleoplasm, chromosomes, and centromeres; plays a role in recruiting CENPE to pericentromeric/centromeric regions during mitosis (PubMed:26321640). Related references: PMID:26321640
• Widely expressed in human tissues, absent in primary spermatocytes; acts as a tumor suppressor and is implicated in developmental disorders (PubMed:12191639). Related references: PMID:12191639

Experimental Guidance and Technical Tips

• Because the immunogen corresponds to the N-terminal region (aa 1-260) conserved in human, mouse, and rat, this antibody may cross-react with these species; verify in your specific sample types.
• For immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), consider nuclear permeabilization and antigen retrieval protocols to enhance signal.
• In chromatin immunoprecipitation (ChIP), the antibody targets abundant CTCF binding sites; optimize crosslinking and shearing conditions to capture robust protein-DNA complexes.
• Expected band size in Western blotting is around 83 kDa; validate with appropriate controls, as post-translational modifications may shift migration.
• ELISA can be employed for high-throughput binding assays; confirm optimal coating buffers and detection systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for IVD raw materials, connecting diagnostic developers with validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For research on chromatin regulation, epigenetics, and transcriptional control, the CTCF Rabbit pAb is one of many high-quality reagents we provide to accelerate your assay development. Our one-stop platform supports every step from concept to clinic with reliable supply chains and technical expertise.