Anti-CTBP2 Polyclonal Antibody for WB, IF/ICC, ELISA - P56545

Core Product Specifications and Parameters

Biological Background: CTBP2 Function and Localization

• Functions as a transcriptional corepressor, regulating diverse transcription factors and playing a critical role in brown adipose tissue (BAT) differentiation.
• Isoform 2 is localized to synapses and likely acts as a scaffold for specialized synaptic structures.
• Ubiquitously expressed, with highest levels in heart, skeletal muscle, and pancreas.
• Possesses NAD-dependent oxidoreductase activity, linking cellular metabolic status to transcriptional repression.
• Subject to post-translational modifications including phosphorylation and methylation, modulating its repressor function.
• Exhibits alternative splicing, generating multiple isoforms with distinct functional roles.
• Localizes to both the nucleus and synapse, reflecting dual involvement in transcription regulation and synaptic organization.
• Implicated in host-virus interactions, suggesting a role in viral processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 345-445 of human CTBP2 (NP_001320.1), a region that may provide isoform specificity.
• For Western blotting, a band at approximately 49 kDa is expected; optimize blocking and detection protocols for this molecular weight.
• The antibody cross-reacts with mouse and rat based on sequence similarity, but validation in these species is recommended.
• In immunofluorescence assays, anticipate nuclear and synaptic staining patterns consistent with CTBP2 localization.
• For ELISA applications, consider appropriate coating conditions and evaluate cross-reactivity with related C-terminal-binding proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to validated IVD raw materials like this CTBP2 antibody, supporting studies in transcriptional regulation, metabolic differentiation, and synaptic biology. As a one-stop sourcing bridge, we offer optimized antibody pairs, bulk reagents, and tailored rare-target procurement to accelerate your assay development from concept to clinic.