Anti-CtBP1 Rabbit Polyclonal Antibody for WB, ELISA - Q13363

Core Product Specifications and Parameters

Biological Background: CtBP1 Function and Localization

• CtBP1 (C-terminal-binding protein 1) is a transcriptional corepressor and short-chain dehydrogenase/reductase, targeting diverse transcription regulators including GLIS2 and BCL6.
• Its dehydrogenase activity utilizes NAD+, contributing to transcriptional regulation and metabolic sensing.
• CtBP1 plays a key role in controlling the equilibrium between tubular and stacked structures in the Golgi complex, impacting membrane trafficking.
• In brown adipose tissue (BAT), CtBP1 is involved in differentiation processes.
• Subcellularly, CtBP1 localizes to both the cytoplasm and nucleus, reflecting its dual functions.
• It is expressed in germinal center B-cells, suggesting roles in immune cell regulation.
• Post-translational modifications such as phosphorylation, ADP-ribosylation, and isopeptide bond formation (potentially sumoylation) regulate CtBP1 activity.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment spanning amino acids 20-250 of human CtBP1, which is expected to generate antibodies recognizing epitopes within the N-terminal region.
• For Western blotting (WB), consider using reducing conditions and validate the antibody in lysates from human or mouse cell lines expressing CtBP1, with a predicted molecular weight of approximately 48 kDa.
• In ELISA applications, the antibody may be suitable for both direct and indirect detection; optimization of coating and detection conditions is recommended.
• Cross-reactivity with human and mouse has been indicated; for other species, perform sequence alignment of the immunogen region to predict reactivity.
• As a polyclonal antibody, lot-to-lot variation can occur; ensure consistency by validating each new lot in your specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with comprehensive access to IVD raw materials, including this anti-CtBP1 polyclonal antibody. With expertise in molecular biology and antibody development, CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support rare target raw-material sourcing to accelerate your research in transcription regulation and cellular processes.