Anti-CTB4a Polyclonal Antibody for WB, ELISA - Q0JF76

Core Product Specifications and Parameters

Biological Background: CTB4a Function and Localization

• CTB4a (also known as LRR-PK1) is a receptor-like kinase from Oryza sativa, characterized by an extracellular leucine-rich repeat (LRR) domain and a cytoplasmic serine/threonine kinase domain.
• Predicted to be a transmembrane protein localized at the cell membrane, with a signal peptide directing its insertion.
• Contains ATP-binding and nucleotide-binding sites within the kinase domain, facilitating phosphotransferase activity.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, with potential ubiquitin-like (Ubl) conjugation.
• Identified through proteomics as a phosphoprotein, indicating its involvement in phosphorylation-mediated signaling.
• Classified under the serine/threonine-protein kinase family, suggesting a role in intracellular signal transduction cascades.

Experimental Guidance and Technical Tips

• For Western blot, consider using total protein extracts from Oryza sativa tissues. The predicted molecular weight is approximately 120 kDa; ensure proper gel percentage and transfer conditions.
• In ELISA, the antibody may be used to detect recombinant or native CTB4a; validate with appropriate positive and negative controls.
• Since the immunogen spans the N-terminal extracellular region, the antibody likely recognizes the native protein under non-reducing conditions.
• Cross-reactivity has been confirmed for Oryza sativa; if testing other species, validate specificity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your procurement of plant biology research reagents by supplying validated antibody pairs, optimized polyclonals like this anti-CTB4a antibody, and bulk ancillary reagents. Our team also assists in sourcing rare targets within plant kinome and receptor-like kinase families, ensuring a seamless supply chain from concept to clinic.