Anti-CSTF2T Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H0L4

Core Product Specifications and Parameters

Biological Background: CSTF2T Function and Localization

• CSTF2T (Cleavage stimulation factor subunit 2 tau variant), also known as CstF-64T or TauCstF-64, is a member of the cleavage stimulation factor complex involved in pre-mRNA 3' end processing.
• It is suggested to play a significant role in AAUAAA-independent mRNA polyadenylation, particularly in germ cells, by directly binding to pre-mRNAs (By similarity).
• The protein is localized to the nucleus, consistent with its role in mRNA processing.
• CSTF2T is annotated as a phosphoprotein and contains RNA-binding repeats, highlighting its regulatory and nucleic acid interaction capabilities.
• It represents a tau variant of the canonical CSTF2 (64 kDa subunit), potentially exhibiting tissue-specific functions in testis or germ cell development.
• UniProt lists the protein under keywords including mRNA processing, Nucleus, Phosphoprotein, and Reference proteome.

Experimental Guidance and Technical Tips

• Given the immunogen targeting the C-terminal region (aa 420-560) of human CSTF2T, the antibody may recognize both endogenous and recombinant forms of the protein.
• For Western blot detection, a band around 64 kDa is expected; consider using reducing conditions and appropriate molecular weight markers.
• Immunofluorescence assays should be optimized with appropriate fixation and permeabilization methods, and include nuclear staining to confirm localization.
• ELISA applications can benefit from using the recombinant immunogen as a standard for quantitative analysis.
• Cross-reactivity with mouse CSTF2T has been reported; validate in your specific tissue/cell line, especially germ cell-related models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents, supporting researchers and diagnostic manufacturers in the mRNA processing and germ cell research areas. Our one-stop sourcing model streamlines procurement from concept to clinic, ensuring reliable access to high-quality antibodies like the anti-CSTF2T polyclonal antibody.