Anti-CSTF2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P33240

Core Product Specifications and Parameters

Biological Background: CSTF2 Function and Localization

• CSTF2 encodes cleavage stimulation factor subunit 2, also known as CstF-64, a 64 kDa subunit of the cleavage stimulation factor complex.
• It is directly involved in binding to pre-mRNAs, playing a critical role in polyadenylation and 3'-end cleavage of mammalian pre-mRNAs.
• Localizes to the nucleus where it participates in mRNA processing.
• Post-translational modifications include phosphorylation and methylation, and the protein is subject to alternative splicing.
• Associated with intellectual disability as a disease variant.
• Contains RNA-binding repeats and is involved in isopeptide bond formation and ubiquitin-like conjugation.
• Identified as a reference proteome component in large-scale proteomics studies.

Experimental Guidance and Technical Tips

• For western blotting, consider using whole-cell or nuclear extracts, as CSTF2 is nuclear-localized. A band around 61 kDa is expected.
• The immunogen spans the N-terminal domain (aa 1-180), which is conserved across human, mouse, and rat, supporting multi-species reactivity.
• For IHC-P, antigen retrieval and nuclear staining patterns may be observed; validate in relevant tissue samples.
• In ELISA, the antibody can be used for detecting recombinant or native CSTF2; consider direct or sandwich formats and validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with comprehensive access to IVD raw materials and technical services. This anti-CSTF2 polyclonal antibody exemplifies our offering in the mRNA processing and polyadenylation research field. From validated antibodies and antigen pairs to bulk ancillary reagents, CamelBio bridges your sourcing needs from concept to clinic.