Anti-CSTF1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q05048

Core Product Specifications and Parameters

Biological Background: CSTF1 Function and Localization

• CSTF1, also known as CstF-50 or CF-1 50 kDa subunit, is a component of the cleavage stimulation factor (CSTF) complex involved in pre-mRNA processing.
• It is one of multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May mediate interaction of CSTF with other factors to form a stable complex on the pre-mRNA. Related references: PMID:10669729
• The protein contains WD repeats, which are often involved in protein-protein interactions.
• CSTF1 is localized to the nucleus, consistent with its role in mRNA processing.
• Keywords associated with this protein include mRNA processing, nucleus, and proteomics identification.

Experimental Guidance and Technical Tips

• This antibody was generated against a recombinant protein covering the N-terminal region (aa 1-220) of human CSTF1, which includes part of the WD repeat domain. This region is conserved across human, mouse, and rat, supporting cross-reactivity.
• For Western blot, the predicted molecular weight is ~48 kDa; consider using appropriate positive controls such as HeLa or HEK293 nuclear extracts.
• For IHC-P, validate in the relevant sample system; CSTF1 is nuclear, so expect nuclear staining. Antigen retrieval may be required.
• For ELISA, optimize coating conditions and antibody dilution using the immunogen or full-length protein as standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a seamless one-stop sourcing platform for IVD raw materials, supporting research and diagnostic development across the entire workflow. For studies on pre-mRNA processing and polyadenylation, we offer validated CSTF1 antibodies, matched antigen standards, and bulk ancillary reagents. Whether you need optimized polyclonal antibodies, monoclonal alternatives, or rare target sourcing, CamelBio connects you with reliable supplies to accelerate your project.