Anti-CSTB Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P04080

Core Product Specifications and Parameters

Biological Background: CSTB Function and Localization

• Cystatin-B (also known as stefin-B, CPI-B, and liver thiol proteinase inhibitor) is a member of the cystatin superfamily of protease inhibitors.
• It functions as an intracellular reversible inhibitor of cysteine proteases, particularly cathepsins L, H, and B.
• The protein localizes to both the cytoplasm and nucleus, consistent with its intracellular inhibitory role.
• CSTB is implicated in neuroprotection; loss-of-function mutations are associated with progressive myoclonus epilepsy type 1 (EPM1), a neurodegenerative disorder.
• Post-translational modifications include acetylation, as identified by proteomics.
• The protein has a molecular weight of approximately 11 kDa and consists of 98 amino acids.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human CSTB (aa 1-98), suggesting the antibody may recognize the native conformation as well as linear epitopes; consider using it for both native and denaturing applications.
• For Western blotting, note the small size of CSTB (~11 kDa); use a 15% SDS-PAGE gel and appropriate transfer conditions (e.g., 0.2 µm PVDF membrane) to ensure retention.
• In immunohistochemistry (IHC-P), CSTB is expected to show cytoplasmic and nuclear staining; validate with a known positive control tissue such as human liver or brain.
• For ELISA, the antibody can be used as either capture or detection after appropriate pairing optimization; consider testing against recombinant CSTB protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-CSTB polyclonal antibody is part of our portfolio for protease-related and neurological disease research. We supply validated antibody pairs, bulk ancillary reagents, and assist with rare target sourcing—ensuring you have reliable materials for your assay development and production needs.