Anti-CSTB Polyclonal Antibody for WB, ELISA - P04080

Core Product Specifications and Parameters

Biological Background: Cystatin-B Function and Localization

• Cystatin-B, also known as Stefin-B, CPI-B, or liver thiol proteinase inhibitor, is encoded by the CSTB gene (also designated CST6, STFB).
• It functions as an intracellular thiol proteinase inhibitor, acting as a tightly binding reversible inhibitor of cathepsins L, H, and B.
• Cystatin-B belongs to the cystatin superfamily, specifically the stefin family of cysteine protease inhibitors.
• Subcellularly, it is found in both the cytoplasm and nucleus.
• The mature protein has a molecular weight of approximately 11 kDa (UniProt: 11140 Da).
• Mutations in CSTB are associated with progressive myoclonus epilepsy (EPM1) and neurodegenerative disorders, as highlighted by the keywords Epilepsy and Neurodegeneration.
• Post-translational modifications include acetylation, and direct protein sequencing evidence is available.
• Additional keywords indicate that 3D structures exist, and the protein is part of the reference proteome with proteomics identification data.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CSTB protein (aa 1-98). Expect detection of endogenous CSTB; validate in relevant human or rat sample systems.
• For Western blot, a band near 11 kDa should be observed. Optimize antibody dilution based on sample type and expression level.
• In ELISA, the antibody can be used as capture or detection; consider pairing with a matched antibody for sandwich assays.
• As CSTB localizes to both cytoplasm and nucleus, use lysis buffers that ensure efficient extraction from both compartments.
• Observed cross-reactivity with rat suggests epitope conservation; sequence alignment may guide use in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, providing validated antibody pairs, optimized polyclonal antibodies like anti-CSTB, and bulk ancillary reagents. Our supply capabilities extend to rare target sourcing, supporting diagnostic manufacturers and researchers in protease inhibition, neurodegeneration, and epilepsy studies. From concept to clinic, we deliver reliable raw materials and expert technical services.