Anti-CST8 Rabbit Polyclonal Antibody for WB, ELISA - O60676

Core Product Specifications and Parameters

Biological Background: CST8 Function and Localization

• Cystatin-8 is a member of the cystatin superfamily of cysteine protease inhibitors, characterized by conserved disulfide bonds and inhibitory activity.
• It is predominantly expressed in the male reproductive tract, with highest levels in the proximal caput region of the epididymis, and lower expression in the testis.
• Within the testis, CST8 localizes to elongating spermatids; in the epididymis, it is exclusively synthesized by the proximal caput epithelium.
• The protein is secreted and contains a signal peptide, consistent with its extracellular role in the epididymal lumen.
• CST8 is predicted to function as a thiol protease inhibitor, potentially protecting sperm from premature proteolysis during maturation and storage.
• The mature protein has a calculated molecular weight of approximately 16 kDa, aligning with the detected band size in Western blotting.
• Glycosylation is likely, as the protein contains potential N-linked glycosylation sites and is annotated as a glycoprotein.
• Its restricted expression pattern suggests a specialized role in sperm development and fertility.

Experimental Guidance and Technical Tips

• For Western blotting, use reducing conditions and validate with human or mouse epididymis/testis lysates; the immunogen corresponds to a region excluding the signal peptide, recognizing the mature form.
• Mouse cross-reactivity allows the antibody to be employed in rodent models, but confirm species-specific reactivity with appropriate controls.
• In ELISA, the recombinant immunogen (aa 53-142) can serve as a positive control; optimize coating conditions and antibody dilution empirically.
• Because this is a polyclonal antibody, lot-to-lot consistency may vary; include internal reference standards in each experiment.
• No quantitative application data are provided; determine optimal working concentrations for your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions for reproductive biology and fertility research, including this anti-CST8 polyclonal antibody. Our catalog includes validated antibody pairs, optimized polyclonals, and bulk ancillary reagents for ELISA and WB workflows. For custom or rare-target sourcing needs, our technical team provides end-to-end support from concept to clinic.