Anti-CST6 (Cystatin-M) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15828

Core Product Specifications and Parameters

Biological Background: Cystatin-M (CST6) Function and Localization

• Cystatin-M (CST6), also known as Cystatin-6 or Cystatin-E, is a secreted thiol protease inhibitor encoded by the CST6 gene.
• It functions as a high-affinity inhibitor for cathepsin L, cathepsin L2 (cathepsin V), and legumain, regulating proteolytic activity in specific tissues. Related references: PMID:30425301
• Involved in the regulation of epidermal cornification, hair follicle morphogenesis and maintenance, playing a critical role in skin barrier formation and cycling. Related references: PMID:30425301
• Tissue expression is restricted to the stratum granulosum of normal skin, the stratum granulosum/spinosum of psoriatic skin, and the secretory coils of eccrine sweat glands; low levels are found in the nasal cavity.
• The protein contains a signal peptide, two intrachain disulfide bonds, and is glycosylated, contributing to its stability and secretory trafficking.
• Mutations in CST6 are associated with ectodermal dysplasia, a condition affecting skin, hair, and nails, underscoring its importance in epidermal development.
• Belongs to the cystatin family of protease inhibitors, characterized by a conserved cystatin domain; it shares homology with other type 2 cystatins but exhibits distinct tissue-specific regulation.

Experimental Guidance and Technical Tips

• For Western blotting, consider that the mature protein migrates at approximately 17 kDa; pre-staining or adjusting blocking conditions may optimize signal detection.
• In immunofluorescence, due to the secreted nature of CST6, detect intracellular pools by permeabilizing cells; co-staining with relevant organelle markers can clarify localization patterns.
• ELISA applications may require optimization of coating buffer and antibody dilution; validate performance in the relevant sample system to ensure specificity.
• As a polyclonal antibody, batch-to-batch variation may influence background; consider titrating the antibody and using appropriate controls to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions for dermatological and genetic disease research, including high-quality antibodies against key skin protease regulators like CST6. Our portfolio spans validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, ensuring reliable supply from concept to clinic. For rare targets, we leverage global sourcing networks to expedite your diagnostic development.